抗坏血酸对铜胁迫下广藿香幼苗生长、铜积累和抗氧化酶活性的影响  

Effects of exogenous ascorbic acid on seedling growth,copper accumulation and antioxidant enzyme activities of Pogostemon cablin under copper stress

作　　者：李贺勤[1] 王维华[1] 江绪文[1] 

机构地区：[1]青岛农业大学农学与植物保护学院/山东省旱作农业技术重点实验室,青岛266109

出　　处：《环境化学》2016年第7期1431-1437,共7页Environmental Chemistry

基　　金：青岛农业大学高层次人才引进项目(1113346);青岛农业大学应用型人才培养特色名校建设工程教学研究项目(XJG2013147);山东省优秀中青年科学家科研奖励基金(BS2014NY007);山东省旱地作物水分高效利用创新团队项目(62112N5)资助~~

摘　　要：通过室内水培试验,研究了外源添加抗坏血酸(Ascorbic acid,As A)对铜(Copper,Cu)胁迫下广藿香(Pogostemon cablin(Blanco)Benth.)幼苗生长、Cu^(2+)积累和抗氧化酶活性的影响.结果显示,与对照相比,10—80μmol·L-1Cu^(2+)降低了广藿香幼苗的苗长、根长、植株生物量和根系耐性指数,且随Cu^(2+)浓度增大抑制作用增强.与Cu^(2+)胁迫相比,外源添加0.10—0.50 mmol·L^(-1)As A提高了广藿香幼苗的苗长、根长、植株生物量、根系耐性指数、根系活力以及SOD、POD、CAT和APX酶活性,降低其根系相对电导率、MDA含量和Cu^(2+)含量;随As A浓度的增加,苗长、根长、植株生物量、根系耐性指数、根系活力以及POD和CAT活性先升高后下降,相对电导率、MDA含量和Cu^(2+)含量先下降后上升,SOD和APX活性则一直增加.结果显示,外源添加0.25 mmol·L^(-1)的As A能提高广藿香幼苗细胞清除活性氧的能力,一定程度上缓解40μmol·L^(-1)Cu^(2+)胁迫对膜系统的伤害,减轻Cu^(2+)胁迫对广藿香幼苗生长的毒害作用.Effects of exogenous ascorbic acid on the growth,copper accumulation and antioxidant enzyme activities of Pogostemon cablin（ Blanco） Benth. seedlings under copper stress were studied by a hydroponic experiment in the room. The results showed that,compared with control,shoot length,root length,biomass and root tolerance index were all reduced by 10—80 μmol·L^-1Cu^2＋.With the increase of Cu^2＋concentration,the inhibitory effects were enhanced. Compared with copper stress,shoot length,root length,biomass,root tolerance index,root vigor and the activities of superoxide dismutase,peroxidase,catalase and aseorbateperoxidase in roots of pogostemon cablin seedling were all improved by exogenous ascorbic acid with 0. 10—0. 50 mmol·L^-1,while relative electrical conductivity,copper accumulation and malondialdehyde content were reduced. With the increase of AsA concentration,shoot length,root length,biomass,root tolerance index,root vigor and the activities of peroxidase and catalase first increased and then decreased. Relative electrical conductivity,copper accumulation and malondialdehyde content first decreased and then increased.The activities of superoxide dismutase and aseorbateperoxidase increased. The above results indicated that the ability of eliminating reactive oxygen species could be improved and the damage of cell membrane system by 40 μmol · L^-1Cu＋2could be alleviated to some degree by exogenous0.25 mmol·L^-1ascorbic acid.

关 键 词：广藿香 铜胁迫 抗坏血酸 幼苗生长 抗氧化酶 

分 类 号：X173[环境科学与工程—环境科学] X53