不同蛋白质源对草鱼和花鲈肉质影响的比较研究  

A Comparative Study: Effects of Different Protein Sources on Flesh Quality of Grass Carp(Ctenopharyngodon idellus) and Japanese Seabass(Lateolabrax japonicas)

作　　者：于晓彤[1] 梁晓芳 王嘉[1] 韩芳[1] 李军国[1] 郑银桦[1] 吴秀峰[1] 王鑫[1] 梁旭方[3] 薛敏[1,4,5] 

机构地区：[1]中国农业科学院饲料研究所,国家水产饲料安全评价基地,北京100081 [2]农业部食物与营养发展研究所,北京100081 [3]华中农业大学水产学院,武汉430070 [4]农业部饲料生物技术重点开放实验室,北京100081 [5]淡水水产健康养殖湖北省协同创新中心,武汉430070

出　　处：《动物营养学报》2016年第7期2055-2068,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点基础研究发展计划(973计划,2014CB138600);国家自然科学基金项目(31372539,31572631);公益性行业(农业)专项经费项目(201203015,201203023);北京市现代农业产业技术体系(SCGWZJ201600031-1)

摘　　要：本试验旨在比较不同蛋白质源对草鱼(Ctenopharyngodon idellus)及花鲈(Lateolabrax japonicas)体成分、肌肉游离氨基酸组成及鱼片质构特性的影响。设计草鱼和花鲈饲料各3种,分别等氮等能。3种草鱼试验饲料分别为以鱼粉为唯一蛋白质源的CI-FM组(对照组),以混合植物蛋白质(豆粕和大豆浓缩蛋白)替代80%鱼粉的CI-PPB80组及以混合植物蛋白质替代100%鱼粉的CI-PPB100组。3种花鲈试验饲料分别为以鱼粉为唯一蛋白质源的LJ-FM组(对照组),以混合植物蛋白质(棉籽浓缩蛋白和大豆浓缩蛋白)替代50%鱼粉的LJ-PPB50组和以混合植物蛋白质替代100%鱼粉的LJ-PPB100组。草鱼初始体重为(153.40±0.30)g,随机分为3组,每组3个重复,每个重复20尾鱼;花鲈初始体重为(12.97±0.03)g,随机分为3组,每组4个重复,每个重复25尾鱼。饲喂8周后,检测2种试验鱼的体成分、肌肉游离氨基酸含量及鱼片质构特性、剪切力、滴水损失及胶原蛋白含量。结果表明:与CI-FM组相比,CI-PPB100组草鱼肌肉粗脂肪含量显著降低(P<0.05),粗灰分含量显著升高(P<0.05);而花鲈肌肉粗脂肪含量则随替代水平的升高呈现先上升后下降的趋势,表现为LJ-PPB50组显著高于LJ-PPB100组(P<0.05)。草鱼肌肉总游离氨基酸及生物胺前体含量各组间无显著差异(P>0.05);随着替代水平的升高,花鲈肌肉总游离氨基酸及呈味氨基酸含量显著降低(P<0.05),生物胺前体含量显著升高(P<0.05)。2种试验鱼肌肉中存在各自的特异性成分。2种试验鱼生、熟鱼片的质构特性差异较大。草鱼的CI-FM组和花鲈的LJ-FM组生鱼片的硬度、黏性、咀嚼力、回弹力及剪切力均分别显著高于同鱼种的其他2组(P<0.05)。对于熟鱼片,草鱼的CI-PPB80组内聚力显著高于其他2组(P<0.05),其他各项指标各组间无显著差异(P>0.05);花鲈的LJ-PPB100组硬度及黏附性显著高于LJ-FM组(P<0.05),其他各项指标各组间无显著差异(P>0.This study aimed to compare the effects of different protein sources on body composition,free amino acid composition in muscle and fillet texture characteristics of grass carp（ Ctenopharyngodon idellus） and Japanese seabass（ Lateolabrax japonicas）. Three isoenergetic and isonitrogenous experimental diets were designed for grass carp and Japanese seabass,respectively. The control group diet for grass carp used low temperature steam-dried fish meal as sole protein source,and used blended plant protein（ soybean meal and soybean protein concentrate） to replace 80% and 100% fish meal,respectively,which were named as CI-FM,CIPPB80 and CI-PPB100 groups,respectively. The control group diet for Japanese seabass used Peru fish meal as sole protein source,and used blended plant protein（ cottonseed protein concentrate and soybean protein concentrate） to replace 50% and 100% fish meal,respectively,which were named as LJ-FM,LJ-PPB50 and LJPPB100 groups,respectively. Each grass carp diet was fed to 3 replicates with 20 grass carp [initial body weight of（ 153.40 ± 0.30） g ],and each Japanese seabass diet was fed to 4 replicates with 25 Japanese seabass[（ initial body weight of（ 12.97±0.03） g]. After an 8-week experiment,body composition,free amino acid composition in muscle and fillet texture characteristics,drop loss,shear force and collagen content were investigated. The results show ed that muscle ether extract content of grass carp in CI-PPB100 group were significantly decreased（ P〈0.05）,while ash content was significantly increased compared with CI-FMgroup（ P〈0.05）.How ever,the muscle ether extract content of Japanese seabass was firstly increased and then down with the replacement level increasing,and show ed LJ-PPB100 group was significantly higher than LJ-PPB50 group（ P〈0.05）. No differences were observed in the muscle total free amino acid（ TFAA） and biogenic precursor contents among all groups of grass carp（ P〈0.05）. W ith the replacement level increasing,the musc

关 键 词：草鱼 花鲈 蛋白质源 鱼粉 混合植物蛋白质 游离氨基酸 肉质 

分 类 号：S963[农业科学—水产养殖]