TBX1基因在透明细胞型肾细胞癌组织中的表达及意义  

Expression of TBX1 Gene in Kidney Tissues in Patients with Clear Cell Renal Cell Carcinoma and Its Clinical Significance

作　　者：姜红堃[1] 田红宇[1] 郭燕平[1] 王妍[2] 张宇曦[3] 姜红[1] 

机构地区：[1]中国医科大学附属第一医院儿科,沈阳110001 [2]中国医科大学附属第一医院病理科,沈阳110001 [3]中国医科大学附属第一医院泌尿外科,沈阳110001

出　　处：《中国医科大学学报》2016年第8期692-694,703,共4页Journal of China Medical University

基　　金：国家自然科学基金(81300130);教育部高校博士点专项基金(20122104110001);辽宁省省直医院改革重点临床科室诊疗能力建设项目(LNCCC-D06-2015)

摘　　要：目的通过检测TBX1基因在透明细胞型肾细胞癌(cc RCC)癌组织中的表达,探讨该基因在cc RCC发病中的分子遗传学机制。方法应用实时定量聚合酶链反应(q RT-PCR)法检测12例cc RCC癌组织及对应的癌旁正常肾组织中TBX1 m RNA表达;Western blot法检测肾组织TBX1蛋白表达。结果与癌旁正常肾组织比较,TBX1 m RNA及蛋白表达水平在cc RCC癌组织中均明显增强(均P<0.05)。结论 TBX1基因过度表达可能为cc RCC发生的一种潜在致病机制。Objective To analyze the expression of TBX1 gene in kidney tissues in patients with clear cell renal cell carcinoma（cc RCC）and investigate its molecular genetics mechanism during the tumor development. Methods Real-time quantitative polymerase chain reaction（q RTPCR）was used to detect the expression of TBX1 m RNA in 12 cases of clear cell renal cell carcinoma tissues and the corresponding normal kidney tissues adjacent to carcinoma.The protein expression of TBX1 was assayed by Western blot in both groups. Results Both TBX1 m RNA level and the protein level were significantly up-regulated in cc RCC tissues compare to those in normal kidney tissues adjacent to carcinoma（all P〈0.05）. Conclusion Over-expression of TBX1 gene might be a potentially pathogenic mechanism of cc RCC.

关 键 词：TBX1基因 透明细胞型肾细胞癌 实时定量聚合酶链反应 Western BLOT 

分 类 号：R737.11[医药卫生—肿瘤]