慢性应激大鼠海马DG区牛磺酸在空间学习记忆过程中的变化  

Change of taurine in hippocampal dentate gyrus during spatiallearning and memory in a rat model of chronic stress

作　　者：张兆一[1] 任美玲[1] 杨朋[1] 李静[1] 吴建龙[2] 陈静伊 金清华[2] 

机构地区：[1]延边大学医学院,吉林延吉133002 [2]延边大学医学院生理学与病理生理学教研室,吉林延吉133002

出　　处：《中国实验诊断学》2016年第6期871-874,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金(31560278);延边大学本科生科研项目(ydbksky201577)

摘　　要：目的观察海马齿状回(Dentate Gyrus,DG)区的牛磺酸(Taurine,Tau)在慢性应激模型大鼠空间学习记忆过程中的变化,初步探讨慢性应激影响大鼠空间学习记忆能力的部分机制。方法 SD雄性大鼠16只,随机分为对照组和慢性应激组。以多种刺激随机应用的方法制备慢性应激大鼠模型,并利用脑部微量透析法和HPLC法实时观察慢性应激大鼠海马DG区细胞外液中Tau的含量。其次,利用Morris水迷宫(Morris Water Maze,MWM)观察大鼠的空间学习记忆能力,并观察海马DG区Tau含量在MWM训练过程中的变化。结果 1)慢性应激组大鼠海马DG区细胞外液中的Tau含量明显高于对照组(P<0.05)。2)MWM的定位航行实验中,两组大鼠的逃避潜伏期均随着训练次数的增加而减少,而慢性应激组大鼠训练第4天的逃避潜伏期明显小于对照组(P<0.05);MWM的空间探索实验中,慢性应激组的穿越原站台区次数显著多于对照组(P<0.05)。3)MWM训练过程中,慢性应激组大鼠海马DG区的Tau含量出现明显的增加(P<0.05),而对照组大鼠海马DG区的Tau含量未出现明显变化(P>0.05),两组间有显著性差异(P<0.05)。结论慢性应激可以提高大鼠的空间学习记忆能力,这可能与海马DG区的Tau含量增加有关。Objective To observe the changes of extracellular level of taurine （Tau） in the hippocampal dentate gyrus （DG） in a rat model of chronic stress during Morris water maze （MWM） test,and primarily investigate the neurochemical mechanism responsible for effect of chronic stress on spatial learning and memory. Methods Totally 16 SD rats were randomly divided into two groups, control group and chronic stress （CS） group. The rat model of CS was prepared by random application of varied stressors,and then the extracellular concentration of Tau in the DG was determined by in vivo microdialysis and HPLC. Next, the spatial learning and memory abilities of rats were assessed by Morris water maze （MWM） ,and the extracellular concentration of Tau in the DG was simultaneously observed. Results 1） Compared with the control group, the extracellular concentration of Tau in hippocampal DG was significantly increased in CS group （P〈0.05）. 2） In the place navigation trial of MWM test,the escape latency decreased in both control and CS groups over the course of the training days,however,on the 4th day,escape latency was significantly shorter in CS group compared with control group （P〈0.05）; in the spatial prove trial of MWM test,the number of platform crossings was markedly increased in CS group compared with control group（P〈0.05）. 3） During MWM test, Tau level in the DG was significantly increased in CS group （P〈0.05） ,while it was not changed in control group（P〈0.05）. Conclusion Chronic stress facilitates the spatial learning and memory in rats,which may be related to the increase of Tau in hippocampal DG region.

关 键 词：慢性应激 海马齿状回 牛磺酸 空间学习和记忆 大鼠 

分 类 号：R338.64[医药卫生—人体生理学]