香菇多糖对溃疡性结肠炎大鼠的改善作用及其机制研究  

Regulatory Effect of Lentinan on Immune Function of Ulcerative Colitis Rats and Its Mechanism Study

作　　者：周泠[1] 唐文台[2] 王钢[3] 

机构地区：[1]遵义医学院附属医院中医肛肠科,贵州遵义563000 [2]遵义医学院附属医院病理科,贵州遵义563000 [3]遵义医学院附属医院中医科,贵州遵义563000

出　　处：《中国药房》2016年第22期3044-3047,共4页China Pharmacy

基　　金：贵州省卫生厅科学技术基金立项项目(No.gzwkj2010-1-055)

摘　　要：目的:研究香菇多糖(LTN)对溃疡性结肠炎(UC)大鼠的改善作用及其机制。方法:将64只SD大鼠随机分为正常组(12只)和造模组(52只)。造模组大鼠采用2,4-二硝基氯苯丙酮和2,4-二硝基氯苯乙醇诱导UC模型。将造模成功的大鼠随机分为模型组、阳性组(柳氮磺胺吡啶片,300mg/kg)和LTN低、中、高剂量组(0.2、0.4、0.8mg/kg),每组10只。各给药组大鼠ig相应药物,正常组和模型组大鼠ig生理盐水,每天1次,连续15 d。检测外周血淋巴细胞(PBMC)中CD_3^+、CD_4^+、CD_8^+水平和结肠组织中肿瘤坏死因子α(TNF-α)、白细胞介素2(IL-2)、IL-4、IL-10水平,并观察结肠组织病理变化。结果:与正常组比较,模型组大鼠PBMC中CD_3^+、CD_4^+、CD_8^+水平和CD_4^+/CD_8^+比值以及结肠组织中IL-4、IL-10水平明显降低,结肠组织中TNF-α、IL-2水平明显升高(P<0.05),结肠组织发生明显溃疡性病变(集中于Ⅱ、Ⅲ级)。与模型组比较,各给药组大鼠PBMC中CD_3^+、CD_4^+、CD_8^+水平,CD_4^+/CD_8^+比值以及结肠组织中IL-4、IL-10水平明显升高,结肠组织中TNF-α、IL-2水平明显降低(P<0.05);结肠组织炎症明显减轻、溃疡形成明显改善。结论:香菇多糖对UC模型大鼠具有一定的改善作用,其机制可能与增强大鼠免疫功能有关。OBJECTIVE：To study the regulatory effect of lentinan（LTN） on immune function in ulcerative colitis（UC） rats and its mechanism.METHODS：64 SD rats were randomly divided into normal group（12 rats） and modeling group（52 rats）.UC model was induced in modeling group by a compound method of 2,4-dinitrochlorobenzene（DNCB） acetone and 2,4-DNCB ethanol.After modeling,rats were randomly divided into model group,positive group（sulfasalazine tablet,300 mg/kg）,LTN low-dose,medium-dose and high-dose groups（0.2,0.4,0.8 mg/kg）,with 10 rats in each group.Treatment groups were given relevant medicine intragastrically,and normal group and model group were given normal saline intragastrically,once a day,for consecutive 15 d.The levels of CD3＋,CD4＋,CD8＋ in peripheral blood lymphocytes（PBMC） and the levels of TNF-α,IL-2,IL-4 and IL-10 in colon were all determined,and the pathological changes of colon tissue were observed.RESULTS：Compared with normal group,the levels of CD3＋,CD4＋,CD8＋,the ratio of CD4/CD8＋,and the levels of IL-4 and IL-10 decreased significantly,while the levels of TNF-α and IL-2 in colon tissue significantly increased（P〈0.05）;obvious ulcerative lesion was observed in colon tissue（concentrated in grade Ⅱ and Ⅲ）.Compared with model group,the levels of CD3＋,CD4＋,CD8＋,the ratio of CD4/CD8＋,and the levels of IL-4,IL-10 increased significantly,while the levels of TNF-α,IL-2 in colon tissue significantly decreased（P〈0.05）;the inflammation of colon tissue was significantly reduced,and the ulcer formation was significantly improved.CONCLUSIONS：LTN has a certain improvement effect on UC model rats,and its mechanism may be related to enhancing the immune function.

关 键 词：香菇多糖 溃疡性结肠炎 免疫功能 大鼠 机制 

分 类 号：R574.62[医药卫生—消化系统]