壳聚糖絮凝—盐诱导双水相萃取分离红葡萄籽中原花青素  

Chitosan-based flocculation and salt induced aqueous two-phase extraction of procyanidins from red grape seeds

作　　者：张喜峰[1,2] 何倩[1] 张斌[1] 王明卉 罗光宏[3,4] 

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]甘肃省河西走廊特色资源利用重点实验室,甘肃张掖734000 [3]河西学院凯源生物技术开发中心,甘肃张掖734000 [4]甘肃省微藻工程技术研究中心,甘肃张掖734000

出　　处：《食品与机械》2016年第6期176-179,共4页Food and Machinery

基　　金：甘肃省中小企业创新基金项目(编号:1047GCCG001);国家级大学生创新创业训练计划项目(编号:201510740005)

摘　　要：以原花青素回收率为主要考察指标,通过正交试验确定壳聚糖絮凝纯化的最佳条件;通过研究盐诱导(盐的种类和浓度)双水相体系对红葡萄籽中原花青素萃取效果,建立盐诱导双水相萃取最佳条件。结果表明:在20mL原花青素提取液中,壳聚糖的加入量2 mL、絮凝温度35℃、提取液pH 4时,葡萄籽原花青素回收率为95.62%;盐诱导双水相萃取分离絮凝后原花青素提取液结果表明,加入K_2HPO_4诱导双水相萃取原花青素效果较好,在2 mL絮凝后原花青素提取液中,K_2HPO_4加入量为0.5g时,原花青素萃取率和分配系数分别为87.2%和1.97。According to the recovery rate of procyanidins,the purification process of procyanidins from red grape seeds by chitosan flocculation was optimized by orthogonal test.After procyanidins in the ethanol aqueous extract solution of red grape seeds was separated into two phases by salts,the partition characteristics of it in two phases were studied.Chitosan flocculation was investigated,and the results were as follows.The optimum flocculation was obtained using 2 mL of chitosan with 20 mL ethanol solution of procyanidins at 35 ℃ and pH 4.The recovery rate of procyanidins reached to 95.62%.Then,the salts inducing aqueous two-phase extraction systems were used to further investigate extraction of procyanidins from the pretreated ethanol solution.The results showed that K_2HPO_4 could be used as a good inducer in ethanol aqueous solution.during the inducing separation phase.When 0.5 g K_2HPO_4 was added into ethanol solution of procyanidins（volume 2 mL）,the partition coefficient and recovery of procyanidins reached to 1.97 and 87.2% respectively.

关 键 词：葡萄籽 原花青素 壳聚糖 盐诱导双水相 

分 类 号：TS261.9[轻工技术与工程—发酵工程]