MiR-125a-5p is Upregulated in Plasma of Residents from An Electronic Waste Recycling Site  

作　　者：Li Ran Qiu Xinghua Yang Qiaoyun Li Keqiu Li Guang 

机构地区：[1]State Key Joint Laboratory for Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering and Center for Environment and Health, Peking University [2]Basic Medical College, Tianjin Medical University

出　　处：《生态毒理学报》2016年第2期134-143,共10页Asian Journal of Ecotoxicology

基　　金：National Natural Science Foundation of China(21322705,41121004,21190051,and 21177091)

摘　　要：The mechanism of health effects caused by organohalogen pollutants, e.g., toxins from electronic waste(e-waste), is poorly understood. We supposed that micro RNAs(mi RNAs), an important post-transcriptional regulator, could play a role in this process. In this study, fasting peripheral blood samples were collected from residents living at an e-waste site in northern China and a nearby reference population. Concentrations of e-waste related organohalogen pollutants in plasma from the exposure group were higher than the corresponding measurement in the reference group. Correspondingly, sixty mi RNAs in plasma showed > 2-fold change between the two groups in microarray analysis. Among them, mi R-125a-5p was confirmed to be upregulated by q RT-PCR and its validated targets were enriched in responses to xenobiotics and cancer related pathways. Furthermore, significant positive correlations were found between levels of mi R-125a-5p in plasma and reactive oxygen species(ROS) in polymorphonuclear neutrophil leukocytes(P < 0.05). These evidences suggested oxidative stress might be an intermediate between e-waste related POPs exposure and alteration of plasma mi RNA.The mechanism of health effects caused by organohalogen pollutants, e.g., toxins from electronic waste（e-waste）, is poorly understood. We supposed that micro RNAs（mi RNAs）, an important post-transcriptional regulator, could play a role in this process. In this study, fasting peripheral blood samples were collected from residents living at an e-waste site in northern China and a nearby reference population. Concentrations of e-waste related organohalogen pollutants in plasma from the exposure group were higher than the corresponding measurement in the reference group. Correspondingly, sixty mi RNAs in plasma showed 2-fold change between the two groups in microarray analysis. Among them, mi R-125a-5p was confirmed to be upregulated by q RT-PCR and its validated targets were enriched in responses to xenobiotics and cancer related pathways. Furthermore, significant positive correlations were found between levels of mi R-125a-5p in plasma and reactive oxygen species（ROS） in polymorphonuclear neutrophil leukocytes（P 0.05）. These evidences suggested oxidative stress might be an intermediate between e-waste related POPs exposure and alteration of plasma mi RNA.

关 键 词：E-WASTE originated POLLUTANTS micro RNA mi R-125a-5p EPIGENETICS OXIDATIVE stress 

分 类 号：X171.5[环境科学与工程—环境科学]