超高压酶解提取猪腿骨胶原蛋白  

Enzymatic Extraction of Collagen from Pig Bone under Ultrahigh Pressure

作　　者：李红霞[1,2] 王安琪[1,2] 杨德庆 肖建喜[1] 

机构地区：[1]兰州大学化学化工学院,甘肃兰州730000 [2]兰州大学萃英学院,甘肃兰州730000 [3]兰州凯兰德科技实业有限公司,甘肃兰州730000

出　　处：《皮革科学与工程》2016年第4期20-25,共6页Leather Science and Engineering

基　　金：中央高校基本科研业务费(lzujbky-2014-73)

摘　　要：我们利用超高压生物反应器,在高压条件下,通过生物酶解的方法,从新鲜的猪腿骨中提取胶原蛋白。我们比较了木瓜蛋白酶、胰蛋白酶、菠萝蛋白酶、胃蛋白酶这四种酶制剂的蛋白提取效率,发现在150 MPa超高压条件下,木瓜蛋白酶的提取效率最高。我们同时利用考马斯亮蓝法和羟脯氨酸法测定蛋白的含量,并优化了各种酶的酶解条件,发现木瓜蛋白酶在温度为45℃,pH为5.0、0.3%的酶量,1∶7的料液比的条件下,表现最佳。超高压生物酶解提供了从猪骨粉中快速高效提取胶原蛋白的一个新途径。The collagen has been extracted from big bone using enzymes in an ultrahigh pressure bioreactor. The extraction efficiency of different enzymes including trypsin, papain, pepsin and bromelain also have been evaluated, and it has been discovered that papain behaved the best. The protein content was measured using both Coomassie light blue and Chloramine T methods, and the enzymatic digestion conditions were optimized. For papain, the optimal condition was 45℃, pH 5.0, 0.3 % enzyme and buffer ratio 1∶7. Enzymatic digestion under ultrahigh pressure provides a very promising approach to extract collagen from pig bone.

关 键 词：木瓜蛋白酶 超高压 猪腿骨 胶原蛋白 紫外分光光度法 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]