RUNX3蛋白在肝癌组织中的表达及与临床病理因素的相关性研究  

A study of the relationship between RUNX3 protein level in hepatocellular carcinoma and clinical pathological factors

作　　者：郑绪斌 许海[2] 李永惠 陈志桥[3] 

机构地区：[1] 武汉市东湖医院消化内科,湖北武汉430000 [2] 湖北中医药大学黄家湖医院,湖北武汉430060 [3] 武汉大学中南医院,湖北武汉430000

出　　处：《徐州医学院学报》2016年第7期429-432,共4页Acta Academiae Medicinae Xuzhou

基　　金：中国博士后科学基金（2013M542498）

摘　　要：目的：研究分析人类runt相关转录因子3（RUNX3）蛋白在肝癌组织中的表达变化及其临床意义。方法采用免疫组化法对84例肝癌组织标本（肝癌组）及40例癌旁组织标本（癌旁组）进行染色观察，比较RUNX3蛋白在2组标本中的表达变化，并分析RUNX3蛋白在肝癌发生、发展中的作用。结果肝癌组织中RUNX3蛋白阳性表达率高于癌旁肝组织（χ^2＝14．849，P＜0．05）。Ⅰ＋Ⅱ期肝癌患者的RUNX3蛋白阳性表达率高于Ⅲ＋Ⅳ期患者（χ^2＝5．601，P＜0．05）；高、中分化患者的RUNX3蛋白阳性表达率为75．56％显著高于低分化患者的17．95％（χ^2＝8．697，P＜0．05）；未发生淋巴结转移患者的RUNX3蛋白阳性表达率高于发生淋巴结转移患者（χ^2＝7．571，P＜0．05）；而不同年龄、性别、肿瘤部位、组织学类型、肿瘤大小、是否存在包膜、是否存在门脉癌栓患者RUNX3蛋白阳性表达率差异无统计学意义（P＞0．05）。结论肝癌组织中RUNX3蛋白表达较癌旁组织下调，并且与肝癌的分期、分化程度、淋巴结转移有关，RUNX3蛋白下调表达与肝癌的发生、发展具有一定的关系。Objective To investigate the level of human runt -related transcription factor 3 （ RUNX3 ） protein in hepatocellular carcinoma and its clinical significance .Methods A total of 84 pathologically diagnosed samples of hepa-tocellular carcinoma （an HCC group） and 40 samples of adjacent tissues （an adjacent group） were selected.Then, im-munohistochemical staining was used to observe the levels of RUNX 3 in both groups .Meanwhile , the role of RUNX3 in the development of hepatocellular carcinoma was analyzed .Results Among 80 HCC samples were 41 （48.81%） posi-tive RUNX3 cases, in contrast with 34 （85.0%） positive RUNX3 cases in 40 samples of adjacent tissues , indicating sta-tistical difference between both groups （χ^2 =14.849,P〈0.05）.HCC patients at Stages Ⅰand Ⅱ produced a positive RUNX3 protein rate of 58.18%, which was significantly higher than 31.03% from patients at Stages Ⅲ＋Ⅳ （χ^2 =5.601,P〈0.05）.The positive RUNX3 protein rate was 75.56%in high and medium differentiated patients , which was remarkably higher than 17.95%in patients with low differentiation （χ^2 =8.697,P〈0.05）.The positive RUNX3 pro-tein rate was 56.92%in patients without lymph node metastasis , which was remarkably higher than 21.05%in patients with lymph node metastasis （χ^2 =7.571,P〈0.05）.No significant difference was found between both groups as to age , gender, tumor site, histological type, tumor size, the presence of capsule, the possibility of changes in a positive RUNX3 protein rate for patients with portal vein tumor thrombus （P〉0.05）.Conclusion The level of RUNX3 protein in HCC tissues is lower than that in the adjacent tissues , which is related to the pathological stage , differentiation degree and lymph node metastasis of HCC .Down-regulation of RUNX3 protein is associated with the development of HCC .

关 键 词：RUNX3 肝癌组织 临床病理特征 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]