气相色谱-串联质谱法测定菠萝蜜中的百菌清、克菌丹和己唑醇残留  

Simultaneous Determination of Chlorothalonil, Captan and Hexaconazole Residues in Jackfruit by Gas Chromatography-tandem Mass Spectrometry

作　　者：李萍萍[1] 程景[2] 张振山[1] 吴学进[1] 

机构地区：[1]中国热带农业科学院分析测试中心,海口571101 [2]黑龙江省农产品质量检验检测中心,哈尔滨150090

出　　处：《农药》2016年第8期593-596,共4页Agrochemicals

基　　金：农业部农产品质量安全风险评估专项(GJFP201600301-1)

摘　　要：[目的]建立同时测定菠萝蜜中百菌清、克菌丹和己唑醇农药残留的气相色谱串联质谱（GC-MS/MS）分析方法。[方法]样品经乙腈提取,采用石墨氨基固相萃取柱浓缩与净化,多反应监测模式（MRM）下进行质谱检测,外标法定量。[结果]百菌清、克菌丹和己唑醇的最低检出限分别为0.002、0.003、0.002 mg/kg,定量限均为0.01 mg/kg。添加质量分数在0.01-0.2 mg/kg时3种供试农药的平均添加回收率为78.3%-86.7%,相对标准偏差（RSD）≤6.2%。[结论]该方法简单可靠、重现性好、灵敏度高,适用于菠萝蜜中百菌清、克菌丹和己唑醇的农药残留检测。[Aims] An analytical method for simultaneous determination of chlorothalonil, captan and hexaconazole residues in jackfi＇uit by gas chromatography-tandem mass spectrometry （GC-MS/MS） was developed. [Methods] The jackfi＇uit samples were extracted with acetonitrile, and then purified and concentrated on carbon/NH2 SPE column. The target compounds were determinated under multiple reaction monitoring （MRM） mode and quantified by external standard method. [Results] The limits of detection for chlorothalonil, captan and hexaconazole were 0.002, 0.003 and 0.002 mg/kg, respectively, and the limits of quantitation were all 0.01 mg/kg. The average recoveries at the fortified levels of 0.01, 0.05 and 0.2 mg/kg were in the range of 78.3 to 86.7%, and the relative standard deviations （RSDs） were less than 6.2%. [Conclusions] The method is simple, reliable, with good reproducibility and sensitivity, which is applicable for analysis of chlorothalonil, captan and hexaconazole residues in jackfi＇uit.

关 键 词：气相色谱-串联质谱 固相萃取 菠萝蜜 农药残留 

分 类 号：S481.8[农业科学—农药学] O657.63[农业科学—植物保护]