小叶金露梅对醛糖还原酶和α-葡萄糖苷酶的抑制作用  

Inhibition of Potentilla parvifolia on alpha-glucosidase and aldose reductase

作　　者：孙玉侠[1] 曾阳[1] 张倩茹[1] 余涛[1] 李美华[1] 赵得萍[2] 

机构地区：[1]青海师范大学生命与地理科学学院青藏高原环境与资源教育部重点实验室,青海810008 [2]中国科学院西北高原生物研究所

出　　处：《北京中医药大学学报》2016年第7期586-589,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：青海省自然科学基金项目(No.2015-ZJ-907);青藏高原药用动植物资源重点实验室项目(No.2014-z-y25)

摘　　要：目的对小叶金露梅不同提取部位的α-葡萄糖苷酶(AG)和醛糖还原酶(AR)抑制活性进行研究,找寻天然的AG和AR抑制剂。方法体外实验通过α-葡萄糖苷酶抑制剂和醛糖还原酶抑制剂筛选模型,对小叶金露梅乙酸乙酯部位(总黄酮)、石油醚部位和水层部位提取物的AG和AR抑制活性进行测定;体内实验进行小叶金露梅总黄酮对小鼠糖耐量的测定。结果小叶金露梅总黄酮部位对AG和AR有较高的抑制活性,而且总黄酮部位抑制AG活性的作用强于阿卡波糖。同时,小叶金露梅总黄酮高、低剂量均能提高小鼠的糖耐量。结论小叶金露梅总黄酮对α-葡萄糖苷酶和醛糖还原酶具有抑制活性。Objective To study the inhibitory effects on α-glucosidase （AG） and aldose reductase （AR） extracted from PotentiUa parvifolia in different solvents, and to find out natural inhibitors of AG and AR. Methods With a combined vitro and vivo experiment design, this study measured the in vitro inhibitory effects of AG and AR extraction from Potentilla Parvifolia in three solvents ： ethyl acetate （ total flavonoids）, petroleum ether and water through AG inhibitors and AR inhibitory activity screening mod- els. In vivo effects of total flavonoids from Potentilla Parvifolia on impaired glucose tolerance of mice were also measured. Results Extraction of Potentilla Parvifolia in total flavonoids demonstrated a stronger in- hibitory effect on AR and AG. Its inhibitory effect on AG was higher than that of acarbose. Meanwhile, both high and low dose of total flavonoids improved glucose tolerance in mice. Conclusion Total fla- vonoids in Potentilla parvifolia exhibits inhibitory effects on AG and AR.

关 键 词：小叶金露梅 Α-葡萄糖苷酶 醛糖还原酶 抑制活性 糖耐量 

分 类 号：R284.2[医药卫生—中药学]