基于TLH重组蛋白单抗的磁性免疫层析试纸条构建  

Establishment of magnetic immunochromatographic test strip based on monoclonal antibody against recombinant protein TLH

作　　者：刘莹莹[1] 翁仕强[1] 卢瑛[1] 赵勇[1] 潘迎捷[1] 

机构地区：[1]上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心,上海201306

出　　处：《上海农业学报》2016年第4期76-80,共5页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2009)第6-1号];上海市科学技术委员会部分地方院校能力建设项目(11310501100);上海市科委工程中心能力提升项目(16DZ2280300)

摘　　要：为了检测不耐热溶血毒素TLH,以His-TLH重组蛋白为抗原免疫BALB/c小鼠,利用杂交瘤技术制备TLH单克隆抗体,然后将纯化后的单抗与磁珠偶联制备免疫磁珠,以此免疫磁珠为标记物构建了TLH重组蛋白的磁性免疫层析检测试纸条。结果表明,该磁性免疫层析试纸条实现了TLH重组蛋白的快速定性和定量检测,具有快速、操作简便、灵敏度高(检测限12.5 ng/mL)等特点。本研究所构建的磁性免疫层析试纸条为重组溶血毒素的快速鉴定、诊断和检测提供了一种新途径。In order to detect thermolabile hemolysin（TLH）,the recombinant protein His-TLH was used to immunize a BALB/c mouse,the monoclonal antibody（MAb） against recombinant protein TLH was prepared by hybridoma technique,and then the purified MAb was conjugated with magnetic beads to prepare immunomagnetic beads（IMBs）,thus a magnetic immunochromatographic test strip（MITS） being established by taking the IMBs as labels for His-TLH.The test result showed that the MITS could be used for qualitative and quantitative assay of recombinant protein TLH,having such advantages as simplicity,rapidity and high sensitivity（the detection limit;12.5 ng/mL）.The developed MITS in this study also provides a new means of rapid identification,diagnosis and detection of recombination hemolysin.

关 键 词：副溶血性弧菌 不耐热溶血毒素 单克隆抗体 磁性免疫层析试纸条 

分 类 号：R446.6[医药卫生—诊断学]