HPLC-DAD同时测定左归丸药液中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸含量  

Simultaneous determination of gallic acid,protocatechuic acid,chlorogenic acid,loganin and ferulic acid of herb liquor of Zuogui pills by HPLC

作　　者：张琳[1] 汪轩 邢婧[1] 尹华[1] 章建华[2] 

机构地区：[1]浙江中医药大学药学院中药标准化研究实验室,杭州310053 [2]浙江中医药大学附属第一医院,杭州310006

出　　处：《药物分析杂志》2016年第8期1364-1369,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：2012年国家自然科学基金项目(批准号:81273772)

摘　　要：目的:建立同时测定左归丸药液中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸5种化学成分含量的分析方法。方法:采用HPLC-DAD多波长切换-梯度洗脱技术。色谱柱:Eclipse XDB-C_(18)(4.6 mm×250 mm,5μm);流动相:甲醇-1%醋酸水溶液,梯度洗脱;流速:1.0 m L·min^(-1);分别以各成分的最大吸收波长为检测波长;柱温:30℃。结果:左归丸药液中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸5种成分均具有良好的线性关系(r≥0.999),平均加样回收率为97.76%~101.8%(RSD<3%,n=6)。3批样品中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸含量分别为0.489 0~0.500 6、0.104 4~0.108 4、0.048 56~0.050 66、1.042~1.061、5.287×10-3~5.369×10^(-3) mg·m L^(-1)结论:该方法经方法学验证,可用于左归丸药液及其制剂的质量控制。Objective： To establish a method for simultaneous determination of gallic acid, protocatechuic acid, chlorogenic acid, loganin and ferulic acid contents in herb liquor of Zuogui pills. Methods： HPLC-DAD and the gradient elution wavelength shifts technique were used in the study. The column was Eclipse XDB-C18 （ 4. 6 mm × 250 mm, 5 μ m ） ; the mobile phase was methanol-1% acetic acid solution at the flow rate of 1 mL· min^-1, the detection wavelength was the maximum absorption wavelength of each ingredient; the oven temperature was 30 qC. Results： The contents of all 5 indicative components in herb liquor of Zuogui Pill had a good linearity （ r t〉 0.999 ）. The average recoveries were 97.76%-101.8% （RSD〈3%, n=6 ）. The contents of gallic acid, protocateehuie acid, chlorogenic acid, loganin and ferulic acid in 3 samples were 0. 489 0-0. 500 6 mg· mL^-1, 0.104 4-0.108 4 mg· mL^-1, 0.048 56-0.050 66 mg·mL^-1, 1.042-1.061 mg· mL^-1, 5.287 × 10^-3-5.369 × 10^-3 mg· mL^-1, respectively. Conclusion： This method has been validated, so it can be used to control the quality of herb liquor of Zuogui Pill and its preparation.

关 键 词：左归丸 没食子酸 原儿茶酸 绿原酸 马钱苷 阿魏酸 熟地 山药 枸杞 山茱萸 川牛膝 鹿角胶 龟板胶 菟丝子 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]