固载脂肪酶纳米TiO_2/PD复合材料合成条件优化及表征  

Characterization and Optimization of Synthesis Conditions of Nano TiO_2/PD Composite Materials for Immobilizing Lipase

作　　者：郑述祥[1] 陶玉贵[1] 朱龙宝[1] 堵国成[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《安徽工程大学学报》2016年第4期30-36,共7页Journal of Anhui Polytechnic University

基　　金：安徽省自然科学基金资助项目(1308085MC51)

摘　　要：利用多巴胺(PD)对纳米二氧化钛进行修饰,制备固载脂肪酶TiO_2/PD复合材料,并通过响应面法对pH值、多巴胺质量浓度、反应时间等合成条件进行了优化,以提高脂肪酶固载率;利用FT-IR、热分析对TiO_2/PD复合材料进行表征.结果表明:在pH值为8.79、多巴胺质量浓度为1.98mg/mL、反应时间为7.42h条件下合成的TiO_2/PD复合材料脂肪酶固载率最高,达到了274.26mg/g;FT-IR表征中在1 277cm-1和1 483cm-1出现的多巴胺特征峰以及热分析表征中多巴胺在300℃左右出现热分解,表明了多巴胺对纳米二氧化钛的修饰.利用多巴胺对纳米TiO_2的表面修饰,有效提高了纳米TiO_2的脂肪酶固载率.The TiO2/PD composite materials for immobilizing lipase were synthesized by using nano TiO2 modified by dopamine.The response surface method was used to obtain the optimal synthesis conditions, including pH,dopamine concentration, reaction time and the characteristics of TiO2/PD.The composite materials were determined by FT-IR and thermal analysis. The results showed that the optimal conditions were as follows.pH 8.79 ;dopamine concentration 1.98 mg/mL;reaction time 7.42 h.On this conditions,the lipaseis immobilization rate of TiO2/PD composite reaches 274.26 mg/g.The surface layer of nano-TiO2 was modified by dopamine using FT-IR and Thermal analysis.The results showed the peaks of dopamine were at 1 277 cm-1 and 1 483 cm 1 and was decomposed about 300 ℃.The nano TiO2 modified by dopamine can improve the lipase immobilization rate effectively.

关 键 词：纳米TIO2 多巴胺 脂肪酶 响应面 表征 

分 类 号：R318.08[医药卫生—生物医学工程]