HPLC法同步测定蛹虫草中虫草素及腺苷的含量  

Synchronous Determination of Cordycepin and Adenosine in Cordyceps Militaris by HPLC

作　　者：李兵[1] 张文成[1] 王冰嵩[1] 金礼强 

机构地区：[1]合肥工业大学农产品生物化工教育部工程研究中心,安徽合肥230009

出　　处：《安徽化工》2016年第4期105-106,109,共3页Anhui Chemical Industry

摘　　要：目的：采用HPLC法同步测定蛹虫草中虫草素及腺苷的含量。方法：采用SGE protecol C18（5μm,250mm×4.6mm）色谱柱,流动相为乙腈-水（5∶95,V/V）,检测波长为260nm,流速1.0mL/min,进样量20μL。结果：虫草素和腺苷分别在16.875-540μg/mL、19.375-620μg/mL范围内呈线性相关,相关系数r分别为0.9998、0.9996。该方法重复性、精密度、稳定性及回收率均符合要求。结论：本法用于同步测定蛹虫草中虫草素及腺苷的含量,具有简便、准确和高效等特点。Objective：this study has been to establish a method for synchronous determination of cordycepin and adenosine in cordyceps militaris. Method： SGE protecol C18（5μm,250mm×4.6mm）chromatographic column was used,with mixtures of acetonitrile and water as mobile phase（5∶95,V/V）,the wavelength was 260 mn,the flow rate was 1.0mL/min,the injection volume was 20μL. Results：the linear range of cordycepin and adenosine is 16.875-540μg/mL,19.375-620μg/mL,respectively. The correlation coefficient r is 0.9998,0.9996 respectively. Repeatability,precision,stability and recovery are favorable. Conclusion：the established method is a simple,accurate and efficient for synchronous determination cordycepin and adenosine in cordyceps militaris.

关 键 词：蛹虫草 虫草素 腺苷 同步检测 

分 类 号：O657.7[理学—分析化学]