Galectin-1 knockdown in carcinoma-associated fibroblasts inhibits migration and invasion of human MDA-MB-231 breast cancer cells by modulating MMP-9 expression  

作　　者：Xue Zhu Ke Wang Kai Zhang Fei Xu Yongxiang Yin Ling Zhu Fanfan Zhou 

机构地区：[1]Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China [2]Department of Pathology, The Affiliated Maternity and Child Health Hospital of Nanjing Medical University, Wuxi 214002, China [3]Save Sight Institute, University of Sydney, Sydney, New South Wales 2000, Australia [4]Faculty of Pharmacy, University of Sydney, Sydney, New South Wales 2006, Australia

出　　处：《Acta Biochimica et Biophysica Sinica》2016年第5期462-467,共6页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China （No. 81300787）, the Natural Science Foundation of Jiangsu Province （Nos. BK2012105 and BK20141103）, the Project of Jiangsu Provincial Commission of Health and Family Planning （No. H201546）, the Major Project of Wuxi Municipal Health Bureau （Nos. ZS201401, Z201508, and MS201404）, and the Project of Wuxi Municipal Science and Technology Bureau （No. CSE31N1520）.

摘　　要：Carcinoma-associated fibroblasts （CAFs） play central roles in facilitating tumor progression and metastasis in breast cancer. Galectin-1 （Gal-1）, a marker of CAFs, was previously reported to be associated with tumorigenesis and metastasis of various types of tumors. The aim of this study is to investigate the role of Gal-1 in CAF-mediated breast cancer metastasis and its underlying molecular mechanisms. Our results showed that CAFs isolated from human breast tumor tissues expressed higher level of Gal-1 compared with paired normal fibroblasts, and the conditioned medium （CM） of CAFs significantly induced the migration and invasion of human MDA-MB-231 breast cancer cells. Knockdown of Gal-1 in CAFs dramatically inhibited CAF-CM-induced cell migration and invasion, probably by inhibiting the expression of matrix metalloprotein 9 （MMP-9）. Our findings dem- onstrate that Gal-1-regulated CAFs activation promotes breast cancer cell metastasis by upregulating MMP-9 expression, which indicated that Gal-1 in CAFs might be a potential novel target for breast cancer therapy.Carcinoma-associated fibroblasts （CAFs） play central roles in facilitating tumor progression and metastasis in breast cancer. Galectin-1 （Gal-1）, a marker of CAFs, was previously reported to be associated with tumorigenesis and metastasis of various types of tumors. The aim of this study is to investigate the role of Gal-1 in CAF-mediated breast cancer metastasis and its underlying molecular mechanisms. Our results showed that CAFs isolated from human breast tumor tissues expressed higher level of Gal-1 compared with paired normal fibroblasts, and the conditioned medium （CM） of CAFs significantly induced the migration and invasion of human MDA-MB-231 breast cancer cells. Knockdown of Gal-1 in CAFs dramatically inhibited CAF-CM-induced cell migration and invasion, probably by inhibiting the expression of matrix metalloprotein 9 （MMP-9）. Our findings dem- onstrate that Gal-1-regulated CAFs activation promotes breast cancer cell metastasis by upregulating MMP-9 expression, which indicated that Gal-1 in CAFs might be a potential novel target for breast cancer therapy.

关 键 词：carcinoma-associated fibroblasts GALECTIN-1 METASTASIS matrix metalloprotein 9 

分 类 号：Q279[生物学—细胞生物学] Q492.6