响应面法优化破囊壶菌油脂的超临界CO_2萃取工艺研究  

Optimization of supercritical CO_2 extraction of oil from thraustochytrids by response surface methodology

作　　者：吴海龙[1] 崔岩[1] 成家杨[1] 

机构地区：[1]北京大学深圳研究生院,广东深圳518055

出　　处：《食品工业科技》2016年第17期232-236,共5页Science and Technology of Food Industry

基　　金：海洋公益性行业科研专项经费项目(201305022);深圳市战略新兴产业发展专项资金(JCYJ20150806112221114);深圳市未来产业专项资金(JCYJ20150626110855791)

摘　　要：在单因素实验的基础上采用响应面法对破囊壶菌Aurantiochytrium sp.油脂的超临界CO2萃取工艺进行优化。优化的工艺条件为:萃取压力30.3 MPa、萃取温度64.4℃、CO2流速56 L/h、萃取时间2 h,在此条件下的油脂提取率达到了74.55%;油脂的主要成分为十六碳烷酸(C16∶0)和二十二碳六烯酸(C22∶6,DHA),含量分别为48.818%和35.120%。萃取出的破囊壶菌油脂成分简单,DHA含量高,可作为保健品生产的重要来源。Based on the single factor experiments, the response surface methodology （RSM）was employed to optimize the conditions of supercritical CO2 extraction of oils from Aurantiochytrium sp..The optimum condition were as follow：extraction pressure of 30.3 MPa,temperature of 64.4 ℃,CO2 flow rate of 56 L/h and extraction time of 2 h,under which the oil extraction rate was 74.55%.The main components of oils were palmitic acid and DHA, which were 48.818% and 35.120% of the total fatty acids, respectively.The extracted oils of Aurantiochytrium sp.had simple composition and high DHA content,which can be used as potential resources for health products.

关 键 词：破囊壶菌 超临界CO2萃取 响应面法 优化设计 油脂提取 

分 类 号：TS224.4[轻工技术与工程—粮食、油脂及植物蛋白工程]