从江香猪CYP2A19基因单核苷酸多态性的群体遗传学分析  

Population genetics of single nucleotide polymorphisms of CYP2A19 gene in Congjiang Xiang-pig

作　　者：杨家大[1] 任琼 吴声榕[3] 

机构地区：[1]凯里学院环境与生命科学学院,贵州凯里556011 [2]贵州省从江县农业局,贵州从江557400 [3]凯里学院图书馆,贵州凯里556011

出　　处：《南方农业学报》2016年第7期1209-1215,共7页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(81460572)

摘　　要：【目的】评估从江香猪CYP2A19基因的纯合度及遗传变异,为开发从江香猪药物代谢模型积累基础资料。【方法】应用SNa Pshot检测方法对141份从江香猪样本CYP2A19基因编码区rs323914689、rs81217981、rs333289891、rs343272409位点及3＇端非翻译区rs338584662和rs321736682位点进行分型分析,并计算从江香猪种群的基因型频率、等位基因频率、有效等位基因数（Ne）、杂合度（He）、多态位点数及多态位点百分比。【结果】除rs333289891位点表现为单态外,其余5个位点均检测到2种或3种基因型,多态位点百分比为83.33%。χ2检验结果显示,5个多态位点均处于Hardy-Weinberg平衡状态（P〉0.05）。5个多态位点的Ne介于1.0071~1.3129,He介于0.0071~0.2057。【结论】不同品系猪种由于遗传背景差异而具有不同的单核苷酸变异,其药物代谢特性也因此存在差异。从江香猪群体纯合度高、遗传稳定性好、变异程度低,是其作为药物代谢动物模型的关键基础。[ Objective ]Homozygositiy and genetic variations of CYP2A19 gene in Congjiang Xiang-pig were assessed, in order to accumulate basic informations for developing drug metabolism model. [Method]A total of 141 Congjiang Xiang-pig samples were collected from Congjiang county, Guizhou. Genotypes of loci rs323914689, rs81217981, rs333289891 and rs343272409 in CYP2AI9 gene coding region and rs338584662 and rs321736682 loci in 3＇-end non-translation regions were detected by SNaPshot method. Then genotype frequencies, allele frequencies, numbers of effective alleles （Ne）, heterozygosities （He）, number and percentage of polymorphie loci of Congjiang Xiang-pig population were calculated. [Result]The results showed that, only rs333289891 locus was monomorphie, other 5 loci were polymorphie, with 2 or 3 genotypes, and the percentage of polymorphic loci was 83.33%. Chi-square test results showed that, all 5 polymorphic loci were in the Hardy-Weinberg equilibrium state （P〉0.05）. In 5 polymorphic loci, Ne ranged from 1.0071 to 1.3129, and He ranged from 0.0071 to 0.2057. [Conclusion]Due to difference in genetic background, different breeds of pigs have different mononueleotide variations, and their drug metabolism characteristics are also different. Cangjiang Xiang-pig can taken as animal model of drug metabolism because it has characteristics of high homozygosity, good genetic stability and low variation.

关 键 词：从江香猪 CYP2A19基因 多态位点 遗传学分析 

分 类 号：S828.89[农业科学—畜牧学]