QuEChERS净化-超高效液相色谱-串联质谱法测定婴幼儿谷类辅助食品中12种真菌毒素  

Determination of twelve mycotoxins in cereal-based complementary foods for infants and young children by ultra high performance liquid chromatography coupled to mass spectrometry using QuEChERS approach

作　　者：苏碧玲[1] 谢维平[1] 欧阳燕玲[1] 陈林刚[1] 

机构地区：[1]泉州市疾病预防控制中心,福建泉州362000

出　　处：《中国食品卫生杂志》2016年第4期467-471,共5页Chinese Journal of Food Hygiene

摘　　要：目的建立QuEChERS净化-超高效液相色谱-串联质谱法测定婴幼儿谷类辅助食品中12种真菌毒素的方法。方法样品用乙腈-水-乙酸（84∶15∶1,V/V）提取,提取液经QuEChERS方法净化,采用Synergi 4μ Fusion-RP C18色谱柱（50 mm×2.0 mm,4μm）进行分离,用超高效液相色谱-串联质谱仪进行检测,基质外标法定量。结果12种真菌毒素在各自的线性范围内线性关系良好,相关系数（r）均不低于0.999,回收率在80.5%-106.4%之间,相对标准偏差在5.3%-9.5%之间。结论该方法快速简便,可用于婴幼儿谷类辅助食品中真菌毒素多残留成分的检测。Objective A method was developed for the determination of twelve mycotoxins in cereal-based complementary foods for infants and young children by ultra high performance liquid chromatography coupled to mass spectrometry using Qu ECh ERS approach. Methods Sample was extracted with acetonitrile-water-acetic acid（ 84 ∶ 15 ∶ 1,V / V）. The extracted solution was purified by Qu ECh ERS approach and separated by using Synergi 4μ Fusion-RP C18（ 50 mm × 2. 0 mm,4 μm） chromatographic column, then was determined by ultra high performance liquid chromatography coupled to mass spectrometry. Matrix-matched calibration was used for the quantification. Results Twelve mycotoxins had good linear relationships in the certain correlation ranges with the correlation coefficients all above0. 999. The average recoveries of 12 mycotoxins at three spiked levels ranged from 80. 5% to 106. 4% with relative standard deviations of 5. 3%-9. 5%. Conclusion The method is rapid,simple and can be used for the determination ofmycotoxins in cereal-based complementary foods for infants and young children.

关 键 词：QUECHERS 超高效液相色谱-串联质谱法 真菌毒素 婴幼儿谷类辅助食品 食品污染物 检测 

分 类 号：R155.5[医药卫生—营养与食品卫生学]