龙须菜非琼胶多糖初级结构及细胞免疫调节活性的研究  

Primary Structural Characterization and Cell Immunomodulatory Activity of a Non-agar Polysaccharide Obtained from Gracilaria Lemaneiformis

作　　者：史晨杉 孙桂清 武瑞霞[1] 龚正斯 王向红[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001 [2]河北省海洋与水产科学研究院,河北秦皇岛063000

出　　处：《现代食品科技》2016年第7期12-17,共6页Modern Food Science and Technology

基　　金：国家科技计划支撑项目(2011BAD13B06)

摘　　要：龙须菜是生产琼胶的重要原料,而其中的非琼胶部分常常被忽略。本文以龙须菜为原料,提取其中的非琼胶多糖,并对其结构及体外生物活性进行初探。采用常规工艺提取得到非琼胶多糖GCP,柱前衍生高效液相色谱法分析GCP主要由半乳糖组成,高碘酸氧化及Smith降解表明单糖基由1→3及1→4位糖苷键链接,I2-KI试验及刚果红试验表明GCP中存在较多的分支和较长的侧链,空间构型表现为单股螺旋或自由卷曲状。利用DEAE-Sepharose Fast Flow柱对其进一步分离纯化,得到分子量为1.05×104U的组分GCP-2,通过脾细胞增殖试验及脾细胞损伤试验探讨GCP及GCP-2的细胞免疫调节活性,结果表明在试验浓度范围内GCP活性略高于GCP-2,GCP对脾细胞的最大增值率为7.45%,脾细胞损伤最大保护率为51.50%。本研究表明龙须菜非琼胶多糖具有较好的细胞免疫调节活性和较高的细胞损伤保护作用,可作为新型药物及保健品研发的重要原料。Gracilaria lemaneiformis is an important source of agar,but its non-agar part is often ignored.A non-agar polysaccharide fraction was isolated from Gracilaria lemaneiformis,and its primary structure and in vitro biological activity were preliminarily explored.The non-agar polysaccharide fraction was isolated through the conventional process and was named GCP.The results of pre-column derivatization and high performance liquid chromatography（HPLC） suggested that GCP was composed of galactose.Periodate oxidation and Smith degradation showed that the monosaccharide group was linked by 1→3 and 1→4 glycosidic bonds.I2-KI test and a Congo red test showed that GCP had a large number of branches and long side chains,and the spatial configuration was a single helix or a coil in its free form.A main component,GCP-2 with a molecular weight of 1.05 × 104 U,was obtained after further purification using DEAE Sepharose Fast Flow（DEAE FF） column chromatography.Splenocyte damage and splenocyte proliferation assays were used to analyze the cell immunomodulatory activities of GCP and GCP-2.GCP showed a slightly higher activity than GCP-2 in the test concentration range.The highest splenocyte proliferation rate and the greatest protection rate for damaged splenocytes by GCP were 7.45% and 51.50%,respectively.This study shows that non-agar polysaccharides have good cellular immunomodulatory activity and a strong cell protective effect.In addition,they could be used as raw materials for production of new drugs and health products.

关 键 词：龙须菜 多糖 结构 生物活性 脾淋巴细胞 

分 类 号：TS254.58[轻工技术与工程—水产品加工及贮藏工程] TS201.4[轻工技术与工程—食品科学与工程]