鼠李糖脂与β-环糊精复合提取预测污染土壤中PAHs的生物有效性  

Prediction of PAHs Bioavailability in Spiked Soil by Composite Extraction with Hydroxypropyl-β-cyclodextrin and Rhamnolipid

作　　者：张亚楠[1,2] 杨兴伦[1] 卞永荣[1] 谷成刚[1] 王芳[1] 王代长[3] 蒋新[1] 

机构地区：[1]中国科学院南京土壤研究所,土壤环境与污染修复重点实验室,南京210008 [2]中国科学院大学,北京100049 [3]河南农业大学资源与环境学院,郑州450002

出　　处：《环境科学》2016年第8期3201-3207,共7页Environmental Science

基　　金：国家自然科学基金项目(41271327;41271464;21377138;41271471);中国科学院南京土壤研究所一三五领域前沿项目(ISSASIP1621;ISSASIP1618)

摘　　要：以添加3种典型多环芳烃(PAHs)菲(Phe)、芘(Pyr)、苯并[a]芘(Ba P)的模拟污染土壤(海南红壤)为对象,研究了鼠李糖脂(RL)与β-环糊精(HPCD)复合提取预测污染土壤中PAHs的生物有效性的可行性.结果表明,当RL浓度大于临界胶束浓度时,由于其胶束增溶作用,PAHs的表观溶解度随RL浓度增加而增加,促使更多的PAHs从土壤固相解吸进入土壤溶液中.通过HPCD/RL复合提取土壤中的PAHs的含量与蚯蚓体内吸收的PAHs的量进行相关性分析,发现两者之间存在显著的正相关关系.但是PAHs蚯蚓吸收量是HPCD单独提取量的2.04倍,是HPCD/RL提取量的1.15倍.结果表明,HPCD/RL比HPCD对PAHs提取更能接近蚯蚓富集.因此,RL的添加在一定程度上增加了HPCD对污染土壤中PAHs生物有效性的预测效果,HPCD/RL复合提取法可以作为一种相对快捷可靠的预测PAHs生物有效性的方法.本研究为复合提取在有机污染物生物有效性评价方面提供一个新的方向,也为有机污染物的风险评估和生物修复提供了一定的技术支持.In this study,composite extraction of hydroxypropyl-β-cyclodextrin（ HPCD） with rhamnolipid（ RL） was selected to assess the bioavailability of polycyclic aromatic hydrocarbons（ PAHs） to earthworms in red soil from Hainan, China, spiked with phenanthrene（ Phe）,pyrene（ Pyr） and benzo（ a） pyrene（ Ba P）. The results showed that when RL was more than the critical micelle concentration,apparent solubility of PAHs increased due to micellar solubilization of RL. So more PAHs were desorbed from solid phase of soil. Real biological experiments showed that there was a good linear relationship between earthworm-accumulated PAHs and HPCD / RL-extracted PAHs（ R_2= 0. 98,n = 15）. However,earthworm-accumulated PAHs was 2. 04 times higher than HPCDextracted PAHs,but only 1. 15 times higher than HPCD / RL-extracted PAHs. This indicated that HPCD / RL was more actual and reliable than HPCD in the assessment of PAHs bioavailability to earthworms. Therefore,the addition of RL,to some extent,enhanced the prediction ability of HPCD in PAHs bioavailability,which might provide a new direction and implications in risk assessment and bioremediation of organic contaminants.

关 键 词：鼠李糖脂 Β-环糊精 蚯蚓吸收 生物有效性 多环芳烃 

分 类 号：X131.3[环境科学与工程—环境科学]