干巴菌废弃料酶水解优化条件及黄酮、多糖含量的研究  

Enzymatic Hydrolysis of Waste Materials from Sparassia Thelephora Ganbajun and Content of Flavonoids/Polysaccharides

作　　者：刘品华[1] 林慧[1] 刘明研[2] 余丽访 杨芬[1] 

机构地区：[1]曲靖师范学院化学化工学院,云南曲靖655011 [2]广西百色农业学校,广西百色533000

出　　处：《西南农业学报》2016年第8期1849-1853,共5页Southwest China Journal of Agricultural Sciences

基　　金：上海师范大学资源化学省部共建教育部重点实验室;曲靖师范学院资源与环境化学重点实验室(SYS2010BX01)

摘　　要：测得野生干巴菌废弃料（干固形物）中总氨基酸态氮为0．376g／100g、总黄酮为6．158mg／g、多糖为1．587g／100g。研究了用木瓜蛋白酶、菠萝蛋白酶水解的优化条件及总黄酮、多糖的变化。结果表明。鲜干巴菌：水=1：4的条件下，酶水解最佳条件为：木瓜蛋白酶（≥800U／mg）≥0．75g／100mL、时间7h、pH值6．5、温度65℃，千圆形物中总氨基酸态氮为0．933g/100g，总黄酮3．868mg/g，多糖1．792g/100g；菠萝蛋白酶（300U／mg）1％-1．25％、时间7h、pH5、温度35℃，千固形物中总氨基酸态氮为0．772g/00g，总黄酮4．968mg／g，多糖2．192g／100g。木瓜蛋白酶表现了较好的水解效果。The contents of amino acid nitrogen, flavonoids, and polysaccharides of waste materials from wild Sparassia Thelephora ganbajim （dry substance） were 0. 376 g/100g, 6. 158 mg/g, and 1. 587 g/100g, respectively. The optimized conditions of enzymatic hydrolysis of the waste materials were studied by papain and bromelain through detection indicator of amino acid nitrogen, then the contents of flavonoids and polysaccharides were researched before and after hydrolysis. The optimal condition of enzymatic hydrolysis was determined as follows ： papain （ ≥ 800 U/mg） ≥0.75 g/10OmL, hydrolysis time： 7 h, pH value =6.5, hydrolysis temperature： 65 ℃. Under the condition, the contents of amino acid nitrogen, flavonoids and polysaccharides in dry substance were 0. 933 g/100g, 3. 868 mg/g and 1. 792 g/100g. Bro- melain （300 U/rag） 1% - 1.25 %, hydrolysis time： 7 h, pH value =5, hydrolysis temperature： 35 ℃. The contents of amino acid nitro- gen, flavonoids and polysacoharides were 0, 772 g/100g, 4. 968 mg/g, and 2. 192 g/100g. Papain exhibits better in the enzymatic hydrolys.

关 键 词：干巴菌 酶水解 氨基酸态氮 黄酮 多糖 

分 类 号：TQ281[化学工程—有机化工]