罗非鱼鱼鳞胶原蛋白水解物对成骨细胞MC3T3-E1增殖分化的影响  

Effect of collagen hydrolysates from tilapia fish scale on the proliferation and differentiation of MC3T3-E1

作　　者：吴谦 申铉日[1] 武林贺 张培[1] 苏玥[1] 

机构地区：[1]海南大学食品学院,海口570228

出　　处：《食品科技》2016年第8期120-124,共5页Food Science and Technology

基　　金：国家"十二五"科技支撑计划项目(2012BAD28B06);国家自然科学基金项目(31260376)

摘　　要：目的:研究罗非鱼鱼鳞胶原蛋白水解物(tilapia fish scale collagen hydrolysate,FH)对小鼠成骨细胞前体细胞MC3T3-E1的增殖和分化的影响。方法:采用噻唑蓝法测定MC3T3-E1细胞的增殖率,用p-nitrophenyl phosphate(p-NPP)法测碱性磷酸酶(Alkaline phosphatase,ALP)的活性,Elisa酶联免疫法检测Ⅰ型胶原蛋白(Collagen type Ⅰ,Col Ⅰ)和骨钙素(Osteocalcin,OCN)的分泌量,茜素红染色法测定细胞外基质矿化结节的数量。结果:与空白对照组相比,FH对细胞的增殖性没有显著的促进作用,但是可以显著提高细胞ALP活性,促进Col Ⅰ和OCN的分泌。此外,FH可以促进细胞矿化结节的生成。结论:FH通过促进成骨细胞MC3T3-E1分化和矿化来提高骨的形成,对开发抗骨质疏松症和骨关节炎等疾病的功能性食品有重要的意义。This study aims to investigate the effect of collagen hydrolysates from tilapia fish scale on the proliferation and differentiation of the osteoblastic cell line MC3T3-E1. The proliferation of MC3T3-E1 cells was determined by Methyl thiazolyl tetrazolium（MTT） assay, the activity of alkaline phosphatase（ALP） was assessed by p-nitrophenyl phosphate（p-NPP）, the secretion of collagen type（COLⅠ）, and osteocalcin（OCN） were detected by enzyme-linked immunosorbent assay（ELISA） and the mineralized nodule of MC3T3-E1 cells was evaluated by staining with Alizarin red S dye. In comparison to the control, results showed that FH could not stimulated osteoblastic proliferation. In addition, doses of 100 μg/mL FH significantly increased alkaline phosphatase（ALP） activity by 1.41-folds in osteoblastic cells, compared with the control. Furthermore, FG upregulated osteoblastic Type 1 collagen（COL Ⅰ）, osteocalcin（OCN） levels as well as promoted bone formation. These results suggest that FH promotes bone regeneration by stimulating differentiation and mineralization of osteoblastic MC3T3-E1 cells. Thus, FH is a potential candidate functional food for osteoporosis prevention.

关 键 词：罗非鱼鱼鳞胶原蛋白水解物 成骨细胞前体细胞MC3T3-E1 增殖 分化 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]