铂微电极快速检测食品中Vc  

Electrochemical determination of vitamin C on platinum microelectrode

作　　者：张俊霞[1] 何保山[1] 张长辉 

机构地区：[1]河南工业大学粮油食品学院,郑州450001 [2]河南医学高等专科学校,郑州451191

出　　处：《食品科技》2016年第8期271-275,共5页Food Science and Technology

基　　金：国家自然基金青年科学基金项目(61301037);河南工业大学科技创新人才培育计划专项(2012CXRC02);河南工业大学青年骨干教师培育计划项目;河南省省属高校基本科研业务费专项资金项目(2014YWQQ05)

摘　　要：以铂微电极(PME)作为工作电极,简化三电极为两电极系统,利用多壁碳纳米管(MWCNTs)具有高比表面积、高催化活性、显著增强响应电流的特性,将分散于N,N-二甲基甲酰胺(DMF)的羧基化MWCNTs修饰于自制PME表面,建立一种新型检测Vc的生物传感器。与常见的宏观电极相比,羧基化MWCNTs修饰的铂丝微电极(DMF/carboxyl/MWCNTs/PME)检测Vc,降低了Vc的氧化电位,增大了氧化峰电流值,提高了电子转移速率,并展现出良好的灵敏度及稳定性。通过一系列检测条件及修饰材料的优化,将DMF/carboxyl/MWCNTs/PME应用于Vc标准液检测中,测得Vc浓度在(1.0×10^(-4)~2.0×10^(-1))mol/L内与氧化电流值呈线性关系,且线性方程为:I(A)=4.19711×10^(-8)+1.27293×10^(-5)C(mol/L),检测限为1.0×10^(-5) mol/L(S/N=3)。DMF/carboxyl/MWCNTs/PME检测实际样品的回收率为97%~113%,验证了DMF/carboxyl/MWCNTs/PME快速检测食品中Vc的可行性。In this study, platinum microelectrode（PME） was used as basal electrode, and the three electrodes systems could be simplified as two electrodes systems. Based on the high specific surface area, high conductivity and significant enhancement in the response current of multi-wall carbon nanotubes（MWCNTs）, a new biosensor for detecting Vc got established which was the self-made PME modified by carboxyl MWCNTs dissolved in N,N-dimethylformamide（DMF）. During the detection of Vc, PME embellished by carboxyl MWCNTs（DMF/carboxyl/MWCNTs/PME） reduced the oxidation potential of Vc, increased the peak currents and accelerate electron transfer rate so as to reducing the pollution of Vc oxidation on the electrode compared with common macroscopic electrodes and showed good sensitivity and stability. With the optimized detection condition and modified materials, Vc concentrations in（1.0×10^-4 -2.0×10^-1） mol/L had a linear relation with oxidation current and the linear equation was：I（A）= 4.19711×10^-8＋1.27293×10^-5C（mol/L） with the limit of detection（LOD）： 1.0×10^-5 mol/L（S/N=3）. When detecting samples, recovery rate was between 97%~113%, which verifying the feasibility of DMF/carboxyl/MWCNTs/PME detecting Vc in foods.

关 键 词：羧基化多壁碳纳米管 铂微电极 VC 生物传感器 

分 类 号：TS207.3[轻工技术与工程—食品科学]