Automatic counting of microglial cell activation and its applications  

作　　者：Beatriz I.Gallego Collado Pablo de Gracia 

机构地区：[1]Instituto de Investigaciones Oftalmologicas Ramon Castroviejo, Universidad Complutense de Madrid, Madrid, Spain [2]Facultad de Optica y Optometria, Departamento de Oftalmologia y Otorrinolaringologia, Universidad Complutense de Madrid, Madrid, Spain [3]Midwestern University, Chicago College of Optometry, Downers Grove, IL, USA [4]Department of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, USA

出　　处：《Neural Regeneration Research》2016年第8期1212-1215,共4页中国神经再生研究（英文版）

基　　金：supported by the Science Foundation of Arizona through the Bisgrove Program to PdG,Grant Number:BSP 0529-13;the Ophthalmological Network OFTARED(RD12-0034/0002);the Institute of Health Carlos III;the PN I+D+i 2008–2011;the ISCIII-Subdireccion General de Redes y Centros de Investigación Cooperativa;the European Programme FEDER;the project SAF2014-53779-R;the project:“The role of encapsulated NSAIDs in PLGA microparticles as a neuroprotective treatment” funded by the Spanish Ministry of Economy and Competitiveness

摘　　要：Glaucoma is a multifactorial optic neuropathy characterized by the damage and death of the retinal ganglion cells.This disease results in vision loss and blindness.Any vision loss resulting from the disease cannot be restored and nowadays there is no available cure for glaucoma; however an early detection and treatment,could offer neuronal protection and avoid later serious damages to the visual function.A full understanding of the etiology of the disease will still require the contribution of many scientific efforts.Glial activation has been observed in glaucoma,being microglial proliferation a hallmark in this neurodegenerative disease.A typical project studying these cellular changes involved in glaucoma often needs thousands of images- from several animals- covering different layers and regions of the retina.The gold standard to evaluate them is the manual count.This method requires a large amount of time from specialized personnel.It is a tedious process and prone to human error.We present here a new method to count microglial cells by using a computer algorithm.It counts in one hour the same number of images that a researcher counts in four weeks,with no loss of reliability.Glaucoma is a multifactorial optic neuropathy characterized by the damage and death of the retinal ganglion cells.This disease results in vision loss and blindness.Any vision loss resulting from the disease cannot be restored and nowadays there is no available cure for glaucoma; however an early detection and treatment,could offer neuronal protection and avoid later serious damages to the visual function.A full understanding of the etiology of the disease will still require the contribution of many scientific efforts.Glial activation has been observed in glaucoma,being microglial proliferation a hallmark in this neurodegenerative disease.A typical project studying these cellular changes involved in glaucoma often needs thousands of images- from several animals- covering different layers and regions of the retina.The gold standard to evaluate them is the manual count.This method requires a large amount of time from specialized personnel.It is a tedious process and prone to human error.We present here a new method to count microglial cells by using a computer algorithm.It counts in one hour the same number of images that a researcher counts in four weeks,with no loss of reliability.

关 键 词：GLAUCOMA glial cells microglial cells automatic counting image processing inner plexiform layer outer plexiform layer bilateral activation 

分 类 号：R775[医药卫生—眼科]