地衣芽孢杆菌Bacitracin A高产工业培养基优化  

Optimization of bacitracin a high- yield industrial fermentation medium

作　　者：徐超[1] 吕凤霞[1] 别小妹[1] 陆兆新[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品工业科技》2016年第18期197-201,207,共6页Science and Technology of Food Industry

基　　金：国家科技部支撑计划(2011BAD23B05)

摘　　要：为了降低杆菌肽工业发酵生产成本,提高有效成分杆菌肽A产量,采用单因素实验,寻找目前发酵培养基中黄豆粉与玉米粉的廉价替代成分,并通过Box-Behnken响应面法优化筛选后工业培养基。结果表明:小麦麸皮可以取代玉米粉与黄豆粉,作为发酵培养基中唯一有机碳氮源,有效降低发酵成本。响应面优化工业培养基为:麸皮50.3 g/L,硫酸铵1.53 g/L,磷酸二氢钾1.43 g/L,在此条件下预测杆菌肽A最大产量为1.70 g/L。经实验验证,杆菌肽A产量可以达到1.78 g/L。使用优化后的培养基,发酵结束后,菌体芽孢数量及转化率较高,芽孢数可以达到1.25×1011CFU/m L。本研究提供的培养基可同时达到高产,降低成本,高芽孢形成率的效果。In order to reduce the cost of bacitracin industrial fermentation, improve the bacitracin a production, single factor experiment was used to looking for cheap ingredients to replace soybean flour and corn flour.And screened industrial culture medium was optimized by the Box-Behnken response surface method.Results showed that,wheat bran could replace corn flour and bean flour,as carbon and nitrogen sources in fermentation medium, reduces the cost of fermentation medium cost.The screened medium was： bran 50.3 g/L, ammonium sulfate, 1.53 g/L,potassium dihydrogen phosphate 1,43 g/L, the predicted maximum yield was 1.70 g/L under this condition.Through certification,Bacitracin A production could reach 1.78 g/L.This paper was found that, using the optimized medium,the ability of produce spore by bacterium was high, the number of spore could reach 1.25 × 10-11 CFU/mL. The culture medium could achieve high yield,cost down and high spore formation rate at the same time.

关 键 词：杆菌肽A 高产 响应面 

分 类 号：TS201.3[轻工技术与工程—食品科学]