复合酶法提取石榴籽多糖的工艺优化  

Optimization of Enzymatic Extraction of Polysaccharides from Pomegranate Seeds with Mixed Enzymes

作　　者：王占一[1] 张立华[1] 王玉海[1] 戴博 郑丹丹[1] 王飞[1] 

机构地区：[1]枣庄学院生命科学学院,山东枣庄277160 [2]空军总医院药学部,北京100142

出　　处：《食品科学》2016年第18期19-25,共7页Food Science

基　　金：2012年度国家星火计划项目(2012GA740049);山东省自然科学基金项目(ZR2013BL018)

摘　　要：探讨应用复合酶法提取石榴籽多糖的最佳工艺条件。以多糖得率为考察指标,在单因素试验基础上,通过D-最优混料试验设计优化复合酶配比,Box-Behnken试验设计优化得出影响因素的最佳参数水平,进而得出最佳工艺条件。结果显示:复合酶最优配比为果胶酶24.2%、纤维素酶67.2%、甘露聚糖酶8.6%;并以此参数为基础,得到复合酶法提取石榴籽多糖的最佳工艺条件为液固比20∶1(mL/g)、介质pH 4.5、酶解温度48.5℃、酶解时间287 min,在此条件下,石榴籽多糖得率为2.83%。In this study, we optimized the conditions for enzymatic extraction of polysaccharides from pomegranate seeds with a mixed enzyme preparation consisting of pectinase, cellulase and mannase. The optimization of the mixing ratio of the three enzymes was performed by the combined use of one-factor-at-a-time method and D-optimal mixture design. Thereafter, the enzymatic extraction conditions were optimized by response surface methodology using Box-Behnken design. The yield of polysaccharides was taken as response for both optimizations. It was shown that the optimal combination of enzymes were 24.2% pectinase, 67.2% cellulase and 8.6% mannose and that the optimal extraction conditions were determined as follows： liquid/solid ratio, 20：1（m L/g）; initial pH, 4.5; hydrolysis temperature, 48.5 ℃; and hydrolysis time, 287 min. Under these conditions, the yield of polysaccharides was 2.83%.

关 键 词：石榴籽 多糖 复合酶法 D-最优混料设计 响应面分析法 提取工艺 

分 类 号：R284.2[医药卫生—中药学]