教酒链霉菌L1105木聚糖酶基因xynA原核表达及诱导产酶优化  

Research on the Prokaryotic Expression of Non-cultured Xylanase Gene and the Conditional Optimization of Enzyme Production

作　　者：熊科[1,2] 崔晓亭 高乐[4,2] 李秀婷[3,2] 

机构地区：[1]北京工商大学北京食品营养与人类健康高精尖创新中心,北京100048 [2]北京工商大学食品质量与安全北京实验室,北京100048 [3]北京工商大学北京市食品风味化学重点实验室,北京100048 [4]北京工商大学北京市食品添加剂工程技术研究中心,北京100048

出　　处：《中国调味品》2016年第9期1-4,23,共5页China Condiment

基　　金：国家自然科学基金(31371723);北京市自然科学基金(5144026)

摘　　要：教酒链霉菌(Streptomyces chartreusis)L1105产木聚糖酶性质优良,具有良好的应用价值。目前,尚未见其酶基因克隆表达的报道。研究将教酒链霉菌L1105木聚糖酶基因xynA克隆、连接、转导至原核表达系统中并研究表达系统在不同的时间、温度及IPTG浓度下诱导表达的产酶活力水平。结果表明:教酒链霉菌L1105木聚糖酶基因原核表达载体成功构建,优化诱导表达的最终条件为诱导25h,诱导温度23℃,加入IPTG浓度为0.7mmol/L,在此条件下,获得最大的酶活力45.07U/mL,较初始酶活值3.94U/mL提高了11.43倍。研究为教酒链霉菌L1105产优良木聚糖酶的工业化应用提供了理论依据。The xylanase produced by Streptomyces chartreusis L1105 possesses excellent properties and good industrial application value. At present, there is no report on the cloning and expression of xylanase gene originated from Streptomyces chartreusis. In this study, the xynA gene of Streptomyces chartreusis L1105 is cloned into vector and transduced into E. coil expression system. Then the conditions of xylanase production are investigated including the expression time, temperature and concentration of IPTG, in order to improve the expression efficiency. The results show that the recombinant expression vector is successfully constructed, and the final conditions of enzyme production are induced by 0. 7 mmol/L of IPTG in 25 h at 23 ℃. The maximum activity is 45.07 U/mL,which is 11.43 times higher than that of the initial activity of 3.94 U/mL. This study has provided a theoretical basis for the industrial application of xylanase originated from Streptomyces chartreusis.

关 键 词：教酒链霉菌L1105 木聚糖酶 基因xynA 原核表达 产酶优化 

分 类 号：TS261.12[轻工技术与工程—发酵工程]