Epimedium koreanum Nakai and its main constituent icariin suppress lipid accumulation during adipocyte differentiation of 3T3-L1 preadipocytes  

作　　者：HAN Yunk-Yung SONG Mi-Young HWANG Min-Sub HWANG Ji-Hye PARK Yong-Ki JUNG Hyo-Won 无 

机构地区：[1]Korean Medicine R&D Center,College of Korean Medicine,Dongguk University,Gyeongju 38066,Republic of Korea [2]department of rehabilitation medicine of korean medicine,college of korean medicine,dongguk university,Gyeongju 38066,Republic of Korea [3]Department of Acupuncture&Moxibustion,College of Korean Medicine,Dongguk University,Gyeongju 38066,Republic of Korea [4]Department of Herbology,College of Korean Medicine,Dongguk University,Gyeongju 38066,Republic of Korea

出　　处：《Chinese Journal of Natural Medicines》2016年第9期671-676,共6页中国天然药物（英文版）

基　　金：supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT,&Future Planning(No.2014R1A1A1003758)

摘　　要：Obesity is associated with a number of metabolic abnormalities such as type 2 diabetes and has become a major health problem worldwide. In the present study, we investigated the effects of Epimedium koreanum Nakai(Herba Epimedii, HE) and its main constituent icariin on the adipocyte differentiation in 3T3-L1 preadipocytes. HE extract and icariin significantly reduced lipid accumulation and suppressed the expressions of PPARγ, C/EBPα, and SREBP-1c in 3T3-L1 adipocytes. They also inhibited fatty acid synthase(FAS), acyl-Co A synthase(ACS1), and perilipin. Moreover, HE extract and icariin markedly increased the phosphorylation of AMPK. These results indicated that HE extract and icariin can inhibit the adipocyte differentiation through downregulation of the adipogenic transcription factors, suggesting that HE containing icariin may be used as a potential therapeutic agent in the treatment and prevention of obesity.Obesity is associated with a number of metabolic abnormalities such as type 2 diabetes and has become a major health problem worldwide. In the present study, we investigated the effects of Epimedium koreanum Nakai（Herba Epimedii, HE） and its main constituent icariin on the adipocyte differentiation in 3T3-L1 preadipocytes. HE extract and icariin significantly reduced lipid accumulation and suppressed the expressions of PPARγ, C/EBPα, and SREBP-1c in 3T3-L1 adipocytes. They also inhibited fatty acid synthase（FAS）, acyl-Co A synthase（ACS1）, and perilipin. Moreover, HE extract and icariin markedly increased the phosphorylation of AMPK. These results indicated that HE extract and icariin can inhibit the adipocyte differentiation through downregulation of the adipogenic transcription factors, suggesting that HE containing icariin may be used as a potential therapeutic agent in the treatment and prevention of obesity.

关 键 词：Epimedium koreanum Nakai Herba Epimedii ICARIIN 3T3-L1 cells OBESITY 

分 类 号：R965[医药卫生—药理学]