酶法制备马里兰烟浸膏的工艺优化及其香味成分分析  

Enzymatic Preparation and Flavor Components Analysis of Maryland Tobacco Concrete

作　　者：许春平[1] 曾颖[1] 申屠洪钎 郑凯 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002 [2]四川中烟工业有限责任公司技术研发中心,四川成都610060 [3]河南中烟工业有限责任公司许昌卷烟厂,河南许昌461000

出　　处：《贵州农业科学》2016年第8期117-121,共5页Guizhou Agricultural Sciences

基　　金：郑州市科技局科技攻关项目"利用低次烟叶制备烟用香料的研究"(10PTGG339-2)

摘　　要：为获得优质马里兰烟浸膏,采用酶法制备浸膏,并对制备工艺进行优化得到最佳提取条件,最后对最优条件下制备的浸膏进行香味成分分析,并与非酶解浸膏进行对比。结果表明:马里兰烟浸膏的最优制备工艺为果胶酶∶纤维素酶=1∶1,pH为5,温度为48℃,液料比为9∶1,该条件下,浸膏得率为31.03%。与非酶方法相比,采用酶解的方法浸膏得率提高13.53%;酶解方法与非酶方法相比,马里兰烟浸膏的挥发性香味物质种类从16种增加到23种;香味成分中如苯甲醇、苯乙酸、β-大马烯酮含量增加,分别提高27.64%,69.68%和97.35%;且浸膏中不再检出烟碱。To obtain the Maryland tobacco concrete with good quality,in this paper,the tobacco concrete was made by enzymatic method,and the extraction procedure was optimized and flavor components of tobacco concrete was analyzed.Results：The optimized conditions included pectinuse∶cellulase=1∶1,pH 5,temperature 48℃,liquid-solid ratio 9∶1,and the concrete yield reached 31.03%.Compared with the non-enzymatic method,the yield of enzymolysis method was increased by 13.53%.Compared with the non-enzymatic method,the number of volatile flavor compounds prepared by enzymolysis method was increased from 16 to 23.The contents of aroma components such as benzyl alcohol,benzeneacetic acid andβ-Damascenone were increased by 27.64%,69.68% and 97.35%,respectively,and no nicotine was detected in the concrete.

关 键 词：马里兰烟 浸膏 酶法 香味成分 GC/MS 

分 类 号：TS49[农业科学—烟草工业]