葡糖醋杆菌RZS01发酵产细菌纤维素的研究  

Fermentation of Komagataeibacter nataicola RZS01 for bacterial cellulose production

作　　者：许云华[1] 马波[1,2] 张衡[2] 罗丽[1] 张雷[1] 郑霞[1] 

机构地区：[1]连云港师范高等专科学校生命科学系,江苏连云港222006 [2]南京理工大学化学生物功能材料研究所,江苏南京210094

出　　处：《中国酿造》2016年第9期38-42,共5页China Brewing

基　　金：国家自然科学基金(21206076);江苏省高校自然科学研究面上项目(16KJB180034);江苏省高等职业院校国内高级访问学者计划项目(2015FX032)

摘　　要：采用葡糖醋杆菌(Komagataeibacter nataicola)RSZ01进行细菌纤维素发酵试验,利用单因素和正交试验对发酵培养基组成进行优化,并研究了菌株保藏时间、接种量和培养基初始p H对BC产量的影响。结果表明,保藏期限在30 d以内的菌种可基本保证BC产量;在接种量为8%、初始pH值为5.2时,最优发酵培养基组成为:葡萄糖2.50%,蔗糖3.00%,玉米浆2.2%,磷酸二氢钾0.35%,硫酸铵0.125%。在此条件下,葡糖醋杆菌RSZ01发酵产细菌纤维素的产量为12.05 g/L。Komagataeibacternataicola RSZ01 was used to bacterial cellulose （BC） fermentation tests. The components of fermentation medium were optimized by single factor and orthogonal experiments, and the effects of strain preservation time, inoculum and medium initial pH on BC yield were researched. The results showed that bacteria can guarantee the BC yield basically within preservation time 30 d. In the conditions of inoculurn 8%, initial pH 5.2, the optimal fermentation medium components were glucose 2.50%, sugar 3.00%, corn starch 2.2%, monopotassium phosphate 0.35% and ammonium sulfate 0.125%, the BC yield with K. nataicola RSZ01 fermentation was 12.05 g/L.

关 键 词：葡糖醋杆菌RSZ01 细菌纤维素 发酵 条件优化 

分 类 号：Q939.97[生物学—微生物学]