纳米载银室温固化PMMA材料对雄性小鼠生殖系统的影响  

Effect of Room Curing PMMA Added Nano-silver Base Inorganic Antibacterial Agent on Reproductive System of Male Mice

作　　者：王玉峰[1,2] 张赐童[1,2] 闫通通 史金先 孙世群[3,2] 王晓容[3,2] 

机构地区：[1]吉林大学口腔医学院,吉林长春130021 [2]吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021 [3]吉林大学口腔医院修复科,吉林长春130021

出　　处：《口腔医学研究》2016年第9期920-924,共5页Journal of Oral Science Research

基　　金：吉林省科技厅科技发展计划项目资助课题(编号:20090462);吉林省发展和改革委员会资助项目(编号:2016C045-2)

摘　　要：目的:研究添加2%浓度纳米载银无机抗菌剂的室温固化型聚甲基丙烯酸甲酯(PMMA)材料对雄性小鼠生殖系统的影响。方法:将雄性昆明小鼠随机分组,动物实验模型建立完成后,处死。计算小鼠体重增长率、睾丸脏器系数,观察睾丸组织的病理学改变,进行精子活动率检测、计数和畸形率计算。采用初级精母细胞染色体畸变实验和精原细胞姊妹染色单体互换(SCE)实验检测实验材料对小鼠初级精母细胞及精原细胞染色体的影响。结果:添加2%浓度纳米载银室温固化PMMA材料对小鼠体重增长率、睾丸脏器系数以及精子计数、活动率和畸形率均无显著性影响,各实验组睾丸组织病理切片与阴性对照组无明显差异。添加2%浓度纳米载银室温固化PMMA材料对小鼠的初级精母细胞染色体无致畸变作用;各实验组精原细胞SCE率与阴性组无显著性差异。结论:添加2%浓度纳米载银室温固化PMMA材料对雄性小鼠生殖系统无显著影响。Objective：To investigate the effect of room temperature curing polymethylmethacrylate（PMMA）material with the addition of 2% nano-silver base inorganic antibacterial agent on reproductive system of male mice.Methods：The male kunming mice were randomly divided into four groups,and were killed after animal models were established.The body weight growth rate and the testis tissue coefficient were calculated,and the pathological changes of testis were observed.The rate of sperm motility was detected.The rate of count and abnormal rate were also calculated.The spermatogonium sister chromatid exchange（SCE）test and primary spermatocyte chromosome aberration test were undertaken respectively to detect the effect of room curing polymethylmethacrylate（PMMA）material added 2% nano-silver base inorganic antibacterial agent on the chromosome of spermatogonium and primary spermatocyte.Results：There was no significant effect of room temperature curing PMMA added 2% nano-silver base inorganic antibacterial agent on the growth rate of body weight and testicular organ coefficient,sperm count,sperm motility and abnormal sperm rate.Testis pathological sections between the experiment groups and negative group were not obvious different.The significant effect of room temperature curing PMMA added 2% nano-silverbase inorganic antibacterial agent on the chromosome of primary spermatocyte was not found.There were no significant differences in SCE frequency between experimental groups and the negative group.Conclusion：Room temperature curing PMMA material added 2%nano-silver base inorganic antibacterial agent has no significant effect on reproductive system of male mice.

关 键 词：纳米载银无机抗菌剂 聚甲基丙烯酸甲酯 染色体畸变 姊妹染色单体互换 

分 类 号：R783.1[医药卫生—口腔医学]