赤散囊菌MAPKs超家族的鉴定及分析  

Genome-wide identification and analysis of MAPKs superfamily in Eurotium rubrum

作　　者：王玉臣[1,2] 谭玉梅[1,2] 陈丽[3] 刘建魁[1,2] 刘永翔[1,2] 刘作易[2,4] 

机构地区：[1]贵州省生物技术研究所,贵州贵阳550006 [2]贵州省农业生物技术重点实验室,贵州贵阳550006 [3]贵州师范大学生命科学学院,贵州贵阳550001 [4]贵州省农业科学院,贵州贵阳550006

出　　处：《菌物学报》2016年第9期1117-1129,共13页Mycosystema

基　　金：贵州省科学技术基金(黔科合LH字[2014]7693;[2014]7688);黔农科院自主创新专项([2014]004;[2011]037)~~

摘　　要：本研究以赤散囊菌Eurotium rubrum全基因组序列为对象,利用HMMER软件构建隐马尔可夫模型(hidden markov models,HMM)结合BLAST的方法鉴定了促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)超家族。通过构建系统发育树对鉴定蛋白进行分析,并利用MEME软件进行了保守性基序的预测及活性位点注释。分析结果表明,赤散囊菌基因组包含了4个MAPK蛋白,分别属于Hog1-type、Mpk C-type、Slt2-type和Fus3/Kss1-type类型;3个MAPK kinase(MAPKK)蛋白,分别属于MKK1-type、Pbs2-type和Ste7-type类型;3个MAPK kinase kinase(MAPKKK)蛋白,分别属于BCK1-type、Ste11-type和Ssk22-type类型。保守性基序分析及注释结果表明,MAPKs超家族蛋白都包含了蛋白激酶活性位点"-D[L/I/V]K-"以及保守性的ATP-binding标签序列。MAPK与MAPKK蛋白分别包含了"-Tx Y-"和"-SD[I/V]WS-"磷酸化位点,且MAPK蛋白还包含一个保守性的common docking基序(CD motif),而MAPKKK蛋白则包含了一个功能不明的保守性基序,其一致性序列为"-GTPYWMAPEV-"。研究结果为揭示MAPKs信号途径在赤散囊菌中参与调控的生物学过程奠定了基础。Mitogen-activated protein kinases（MAPKs） have been considered as a family of serine/threonine protein kinases, which are involved in the transduction of a variety of extracellular signals, regulating growth and differentiation processes. To understand the stress-tolerance mechanism of Eurotium rubrum, members of mitogen-activated protein kinase（MAPK） superfamily were identified from E. rubrum genome using the Hidden Markov Models and BLAST method. The phylogenetic trees of the identified proteins of MAPK superfamily were constructed and the conserved motifs were predicted using MEME suite software. The E. rubrum contains four MAPK proteins, namely Hog1-type, Mpk C-type, Slt2-type and Fus3/Kss1-type, three MAPK kinase（MAPKK） proteins, namely MKK1-type, Pbs2-type and Ste7-type, and three MAPK kinase kinase（MAPKKK） proteins, namely BCK1-type, Ste11-type and Ssk22-type. Prediction of the conserved motifs indicates that all of the MAPKs superfamily proteins contain protein kinase activity site ＂-D[L/I/V]K-＂ and ATP-binding site. The MAPK and MAPKK proteins contain ＂-Tx Y-＂ and ＂-SD[I/V]WS-＂ dual phosphorylation site respectively, and the former contains a conserved common docking motif（CD motif）. In addition, all of the three MAPKKK proteins possess a conserved motif ＂-GTPYWMAPEV-＂ with unknown function. Our results could lay a foundation for elucidation of MAPK signal pathway-mediated cellular processes in E. rubrum.

关 键 词：赤散囊菌 促分裂原活化蛋白激酶(MAPK) MAPK激酶(MAPKK) MAPK激酶激酶(MAPKKK) 

分 类 号：Q949.32[生物学—植物学]