超高效液相色谱-三重串联四级杆质谱法测定乌梅中19种深色染色色素  

Detection of 19 dark pigments illegally added in Mumu Fructus by ultra high performance liquid chromatography-tandem mass spectrometry

作　　者：胡青[1] 林媛[2] 张甦[1] 郑希望 崔亚君[2] 季申[1] HU Qing LIN Yuan ZHANG Su ZHENG Xi-Wang CUI Ya-Jun JI Shen(Shanghai Institute for Food and Drug Control, Shanghai 201203, China Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China)

机构地区：[1]上海市食品药品检验所,上海201203 [2]上海中医药大学,上海201203

出　　处：《食品安全质量检测学报》2016年第7期2663-2670,共8页Journal of Food Safety and Quality

基　　金：上海市科学技术委员会项目(14DZ2294000)~~

摘　　要：目的采用超高效液相色谱-三重串联四级杆质谱法建立乌梅中蓝色、绿色、黑色等多种色素检测方法。方法药材经甲醇-0.1%甲酸水（V：V=3：2）超声提取3次,提取液合并,分别经弱阴离子WAX固相萃取柱和弱阳离子WCX固相萃取柱净化得到水溶性酸性色素待测溶液和水溶性碱性色素待测溶液,最后采用超高效液相色谱-三重串联四级杆质谱仪分析测定。结果 19种色素的加标回收率62.3%-128.2%,方法检测限为0.5-345.5μg/kg。将本方法用于实际样品分析,检出部分乌梅有非法染色。结论此方法灵敏、快速,可用于测定乌梅非法染色。Objective To establish a method for detection of 19 dark pigments illegally added in Mumu Fructus by ultra high performance liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS）. Methods Samples were extracted with methanol-0.1% formic acid solution （V：V=3：2） by ultrasonic 3 times, cleaned up with WAX and WCX columns to obtain acidic and alkaline pigments tested solution, respectively. Finally, the analytes were detected by UHPLC-MS/MS. Results Recoveries of 19 kinds of dark pigments in Mumu Fructus were 62.3% - 128.2%, and the limits of detection were 0.5 - 345.5 μg/kg. This method could be applied to sample analysis, and the results found some partial illegally added pigments in Mumu Fructus. Conclusion The established method is fast and sensitive, which can meet the determination requirements of dark pigments illegally added in Mumu Fructus.

关 键 词：乌梅 深色色素 染色 超高效液相色谱-三重串联四级杆质谱法 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]