气相色谱法分析不同产地辣木叶中植物甾醇的含量  

Content determination of phytosterol in Moringa oleifera leaves from different areas by gas chromatography

作　　者：杨扬[1] 赵志红[1] 李小佳[1] 朱慧[1] YANG Yang ZHAO Zhi-Hong LI Xiao-Jia ZHU Hui(Key Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou Wahaha Group Co., Ltd., Hangzhou 310018, China)

机构地区：[1]杭州娃哈哈集团有限公司浙江省食品生物工程重点实验室,杭州310018

出　　处：《食品安全质量检测学报》2016年第8期3179-3183,共5页Journal of Food Safety and Quality

摘　　要：目的研究西双版纳、韶关、安哥拉、印度和云南辣木叶中菜油甾醇、β谷甾醇和豆甾醇的含量差异。方法样品经50%氢氧化钾皂化后,用乙醚：正己烷（1：1,V：V）萃取游离甾醇并将溶剂蒸干,用N,N-二甲基甲酰胺：六甲基二硅氨烷：三甲基氯硅烷（8：2：1,V：V：V）将游离甾醇硅烷化,然后进行气相色谱分析,并分析各地辣木叶中菜油甾醇、豆甾醇和β谷甾醇含量。结果β谷甾醇在韶关辣木叶中含量为20 mg/100 g左右,在西双版纳辣木叶中含量为40 mg/100 g,在安哥拉、印度和云南辣木叶中含量为30 mg/100 g左右;菜油甾醇在韶关辣木叶中含量为5-7 mg/100 g,在印度辣木叶中含量为8 mg/100 g,在西双版纳、安哥拉和云南辣木叶中均大于10mg/100 g;豆甾醇在各个辣木叶中含量为2-12 mg/100 g。结论西双版纳、云南、安哥拉、印度辣木叶中菜油甾醇、β谷甾醇含量明显高于韶关辣木叶,豆甾醇含量没有明显的地域差异。Objective To study the content difference of campesterol, β-sitosterin, and stigmasterol in Moringa oleifera leaves from Xishuangbanna, Shaoguan, Angola, India and Yunnan. Methods After saponification by 50% potassium hydroxide, the free phytosterol of samples were extracted by diethyl ether：hexane（1：1,V：V）, and the solvent were evaporated to dryness before silanization using N,N-Dimethylformamide（DMF）, hexamethyldisilazane（HMDS） and trimethyl-chlorosilane（TMCS）（8：2：1,V：V：V）. The content of campesterol, stigmasterol and β-sitosterin in Moringa oleifera leaves from various areas were analyzed by gas chromatography（GC）. Results The content of β-sitosterin were around 20 mg/100 g in Moringa oleifera leaves from Shaoguan, 40 mg/100 g in that from Xishuangbanna and 30 mg/100 g in those from Angola, India and Yunnan. The content of campesterol were around 5-7 mg/100 g in Moringa oleifera leaves from Shaoguan, 8 mg/100 g in that from India and larger than 10 mg/100 g in those from Xishuangbanna, Angola and Yunnan. The content of stigmasterol were 2-12 mg/100 g in Moringa oleifera leaves from different areas. Conclusion The content of campesterol and β-sitosterin of Moringa oleifera leaves from Xishuangbanna, Yunnan, Angola and India are remarkably higher than those from Shaoguan. The content of stigmasterol has no obvious regional differences.

关 键 词：气相色谱 辣木叶 植物甾醇 

分 类 号：O657.71[理学—分析化学]