米根霉两段式利用板栗栗苞生产富马酸  

Fumaric acid production from autohydrolysis-pretreated Chinese chestnut shell by Rhizopus oryzae in a two-stage fermentation

作　　者：李鑫[1,2,3] 周瑾[3] 虞慧[3] 欧阳水平 LI Xin ZHOU Jin YU Hui OUYANG Shuiping(Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China Key Laboratory of Forest Genetics ＆ Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037,China College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China)

机构地区：[1]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037 [2]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037 [3]南京林业大学化学工程学院,江苏南京210037

出　　处：《生物加工过程》2016年第4期1-4,11,共5页Chinese Journal of Bioprocess Engineering

基　　金：江苏省自然科学基金(BK20131426);江苏省高校自然科学研究重大项目(14KJA220003);江苏省重点研发计划项目(BF2015007)

摘　　要：通过自水解预处理板栗栗苞,以预水解液组成增殖培养基培养米根霉,增殖的米根霉再利用栗苞酶解液生产富马酸。结果表明:220℃自水解预处理栗苞,有效疏解栗苞紧密的木质纤维结构,以50 FPIU(以1 g纤维素计)纤维素酶水解50 g/L预处理栗苞,酶解得率大于95%;经增殖培养基培养米根霉,菌体生物量达4.5 g/L;增殖的米根霉利用栗苞酶解液发酵产富马酸,富马酸质量浓度为15.78 g/L,糖酸转化率为0.34 g/g。通过两段式发酵工艺,米根霉有效利用板栗栗苞生产富马酸。Fumaric acid production from autohydrolysis？pretreated Chinese chestnut shell by Rhizopus oryzae was investigated in a two？stage fermentation. Autohydrolysis pretreatment of Chinese chestnut shell was carried out at 220 ℃. Enzymatic hydrolysis yield of pretreated Chinese chestnut shell was over 95%based on 50 g/L substrate loading using a cellulase loading of 50 FPIU/g cellulose. Rhizopus oryzae could grow and obtain 4. 5 g/L fungal biomass in proliferation medium containing xylose.Pre？cultured Rhizopus oryzae was used for production of fumaric acid from enzymatic hydrolysate of pretreated Chinese chestnut shell. Fumaric acid of 15. 78 g/L was obtained after 96 h fermentation with a yield of 0. 34 g/g.

关 键 词：板栗栗苞 自水解预处理 米根霉 两段式发酵 富马酸 

分 类 号：TQ353.6[化学工程]