浊点萃取-火焰原子吸收检测红葡萄酒中铜、锌、镉  

Determination of Copper, Zinc and Cadmium by Cloud Point Extraction-Flame Atomic Absorption Spectrometry in Red Wine

作　　者：郭金英[1] 李丽[1] 李彤辉[1] 任国艳[1] 王萍[1] 刘建学[1] 张玉先[1] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023

出　　处：《中国食品学报》2016年第7期252-258,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：河南省教育厅自然科学研究计划项目(2011A330001)

摘　　要：建立浊点萃取-火焰原子吸收富集检测红葡萄酒中铜、锌、镉3种金属离子的方法。研究1-2-吡啶偶氮-2-萘酚（PAN）用量、静止时间、硼砂缓冲液用量、Triton X-114用量、萃取液p H值和离心时间等萃取条件对3种金属离子富集效果的影响。分析仪器狭缝宽度、空气乙炔燃烧比和燃烧高度对3种金属离子测定结果的影响。结果表明,浊点萃取的适宜条件为：PAN用量0.50 m L,Triton X-114用量0.50 m L,萃取液p H 7.0,缓冲液用量1.00 m L,静置时间25 min,离心时间5 min。优化的原子吸收测定条件为：狭缝宽度0.7 H,空气乙炔燃烧比2.0∶17.0,燃烧高度0.01 mm。浊点萃取-火焰原子吸收光谱法检测红葡萄酒中铜、锌、镉金属离子的检测限为（0.02±0.001）μg/m L,相对标准偏差为6.70,加标回收率在95.50%~104.00%之间。建立了用浊点萃取-火焰原子吸收联用检测红葡萄酒中铜、锌、镉金属离子含量的方法。此方法简单、准确、方便,适合样品批量测定。Copper, zinc and cadmium are nutritionally essential metals and are widely distributed in nature. These metals play an important role in human metabolism, and are an essential micronutrient required for good health. However, high levels of these elements can be harmful, which can lead to biological disorder. Due to these reasons, the accurate and precise determinations of trace metals of red wine are important. PAN volume, p H, Triton X-114 volume, the centrifugal time, the silent time, the buffer solution were optimized in the cloud point extraction. Appropriate measurement parameteres were established by flame atomic absorption spectrometry of detecting copper, zinc and cadmium.The cloud point extraction optimum condition： p H 7.0, PAN volume 0.5 m L, Triton X-114 volume 0.5 m L, the centrifugal time 5 min, the silent time 25 min after adding the PAN, the buffer solution volume 1.0 m L. Copper, zinc and cadmium detection of red wine by the flame atomic absorption spectrometr： the slit width 0.7 H, the air acetylene ratio 2.0：17.0,the observed altitude 0.01 mm. The limit of detection, relative standard deviation and the recovery rate of the proposed method were（0.02 ± 0.001） μg/m L, 6.7, 95.50%-104.00%, respectively. This method was efficient, sensitive and suitable for large scale determination.

关 键 词：浊点萃取 火焰原子吸收 铜 锌 镉 

分 类 号：O657.31[理学—分析化学]