miR-126调节骨髓间充质干细胞对游离皮瓣组织学活性的作用  

Micro RNA-126 effects on free flap activity by regulating bone marrow mesenchymal stem cells

作　　者：陈勇[1] 范龙坤[1] 

机构地区：[1]河北省沧州市中心医院(河北医科大学沧州临床医学院)口腔颌面外科,河北省沧州市061001

出　　处：《中国组织工程研究》2016年第36期5332-5337,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:miR NA具有组织特异性及细胞特异性,其中内皮细胞特异性高表达miR NA-126(miR-126)在血管新生中具有举足轻重的作用。目的:研究miR-126调节游离皮瓣移植后皮瓣成活以及组织学活性的影响,探讨其在血管新生中的作用机制。方法:(1)利用瞬时转染技术提高骨髓间充质干细胞中mi R-126的表达,利用Real-Time PCR检测巨噬细胞炎性蛋白1α、肿瘤坏死因子α及血管细胞黏附分子1 mR NA表达及Western blot检测ERK1/2、AKT、p ERK1/2、p AKT蛋白表达;(2)将48只SD大鼠随机分为3组,每组16只,均制备腹部游离皮瓣模型。A-C组分别在游离皮瓣远端1 cm和3 cm处注射miR-126 mimics转染的骨髓间充质干细胞、miR-126 mimics control转染的骨髓间充质干细胞及PBS。结果与结论:(1)转染miR-126 mimics后的骨髓间充质干细胞较对照组骨髓间充质干细胞中巨噬细胞炎性蛋白1α、肿瘤坏死因子α及血管细胞黏附分子1 mR NA表达量分别下降36倍、3.5倍和14倍;(2)A组大鼠皮瓣远端组织中ERK1/2、AKT、pE RK1/2、pA KT的蛋白表达水平比B和C组明显增高,且p AKT/AKT及p ERK1/2/ERK1/2比值高于B和C组,且皮瓣组织液中巨噬细胞炎性蛋白1α、肿瘤坏死因子α及血管细胞黏附分子1蛋白水平明显低于B和C组;(3)结果提示miR-126可以促进游离皮瓣组织中血管新生腺管蛋白的活化,进而促进皮瓣的成活。BACKGROUND： MicroR NA has tissue and cell specificity, and high expression of endothelial cell-specific micro RNA-126（mi R-126） plays an important role in angiogenesis. OBJECTIVE： To explore the effect of mi R-126 on transplanted free flap survival and histological activity as well as its mechanism in angiogenesis. METHODS： Transient transfection technology was used to enhance the expression of miR-126 in bone marrow mesenchymal stem cells. Expression levels of macrophage inflammatory protein-1α, tumor necrosis factor-α and vascular cell adhesion molecule-1 mR NA were detected by real-time PCR, and expression levels of ERK1/2, AKT, pE RK1/2, pA KT protein were measured by western blot assay. Forty-eight Sprague-Dawley rats were randomly divided into three groups（n=16 per group）, followed by preparation of abdominal free flap models. Rats in the three groups were given injection of miR-126 mimics-transfected cells, miR-126 mimics control-transfected cells and PBS 1 cm and 3 cm distal to the free flap, respectively. RESULTS AND CONCLUSION： Expression levels of macrophage inflammatory protein-1α, tumor necrosis factor-α and vascular cell adhesion molecule-1 m RNA in the cells transfected with mi R-126 mimics were decreased by 36, 3.5 and 14 times compared with those in the PBS group, respectively. Expressions levels of ERK1/2, AKT, pE RK1/2, p AKT protein in the distal free flap increased significantly in the mi R-126 mimics group than the other two groups, as did the ratios of pA KT/AKT and p ERK1/2/ERK1/2. In addition, the expression levels of macrophage inflammatory protein-1α, tumor necrosis factor-α and vascular cell adhesion molecule-1 protein in the flap tissue fluid were significantly lower in the mi R-126 mimics transfection group than the other two group. All these findings suggest that mi R-126 can promote free flap survival by creating favorable conditions for angiogenesis in the free flap tissue.

关 键 词：外科皮瓣 转染 组织工程 干细胞 骨髓干细胞 游离皮瓣 MIR-126 骨髓间充质干细胞 瞬时转染 巨噬细胞炎性蛋白1Α 肿瘤坏死因子α 血管细胞黏附分子1 血管生成 

分 类 号：R394.2[医药卫生—医学遗传学]