单增李斯特菌便捷检测试纸的制备及检测效果  

Preparation and Applicability of a Simple and Fast Test Strip to Detect Listeria monocytogenes

作　　者：郑晓风[1] 韩勇 刘英玉[1] 张军涛[1] 张琦[1] 张晓红[1] 姚刚[1] 

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [2]新疆乌鲁木齐市畜牧草原工作站,新疆乌鲁木齐830000

出　　处：《食品科学》2016年第20期191-197,共7页Food Science

基　　金：新疆维吾尔自治区高技术发展计划项目(201411105);新疆维吾尔自治区研究生教育创新计划-新疆研究生科研创新项目(XJGRI2015093)

摘　　要：目的:研究一种快速、便捷检测食品中单增李斯特菌的方法,拟对本实验室制备的单增李斯特菌快速检测试纸(Listeria monocytogenes-fast test paper,LM-FTP)(专利受理号:201510274726.9)进行检测效果评价。方法:采集肉样、乳样、水样和蔬菜,用LM-FTP进行检测,并与市售单增李斯特菌检测试纸片(3M Petrifilm^(TM))进行比对实验。结果:LM-FTP的检测特异性为100%,检测限为2.1×10~4 CFU/m L。增菌培养到报告结果只需5.5 h。LM-FTP和3M Petrifilm^(TM)检测都可对样品完成检测,对农贸市场畜产品及蔬菜检测,二者符合率为75%,对超市同类食品检测,二者符合率为62.5%;对超市熟食检测时,二者符合率为93.75%。结论:与市售3M Petrifilm^(TM)检测方法相比,LM-FTP检测试纸制备简单,操作便捷,较3M Petrifilm^(TM)的24 h报告结果更节省时间,但在检测灵敏度方面有一定差距,尚需进一步研究改进。Purpose： In the present study, a paper-based test strip for the fast and simple detection of foodborne L.monocytogenes （LM） was prepared and named as LM-FTP （Patent Acceptance Number： 201510274726.9）, and itsperformance was evaluated. Methods： The performance evaluation was carried out in comparison with 3M PetrifilmTM byusing them to detect samples of meat, milk, drinking water and vegetable collected from farmer’s market and supermarket.Results： The specificity of LM-FTP was 100% with a limit of detection of 2.1 × 104 CFU/mL, and the detection process frombacterial enrichment to obtainment of analytical results took about 5.5 hours. Both LM-FTP and 3M PetrifilmTM could enablethe detection of the samples we tested. The coincidence rate between the results obtained using LM-FTP and 3M PetrifilmTMfor the detection of livestock products and vegetables from farmer’s market was about 75%, whereas that for those fromsupermarket was about 62.5%. In addition, the coincidence rate between the results of both methods for supermarket deliswas about 93.75%. Conclusions： LM-FTP is simpler to prepare, easier to use, and time saving compared with 3M PetrifilmTM,but it was inferior to 3M PetrifilmTM in terms of sensitivity. Therefore, LM-FTP remains to be further improved.

关 键 词：单增李斯特菌 酶底物法 快速检测试纸 

分 类 号：S852.612[农业科学—基础兽医学]