绿咖啡豆中绿原酸的分离纯化  

Studies on separation and purification of chlorogenic acid from green coffee beans

作　　者：张由明 章银军[1] 沈雪亮[1] 

机构地区：[1]浙江工业大学生物工程学院,浙江杭州310014

出　　处：《发酵科技通讯》2016年第3期140-146,共7页Bulletin of Fermentation Science and Technology

摘　　要：对未烘焙绿咖啡豆中绿原酸的分离纯化进行深入研究。采用热水重复分批浸提法,在单因素实验基础上,采用Box-Behnken中心组合实验设计响应面分析法,获得了绿咖啡豆中绿原酸的最佳提取条件:温度79.8℃,溶剂p H值2.8,料液比1∶6,提取时间3.0 h,绿原酸的最大提取得率为92.0%。比较了多种不同规格的树脂对绿原酸的分离纯化效果,最终选用XDA-8大孔树脂,在优化后的最适工艺条件下,绿原酸含量可达70.9%,回收率达85.4%。进一步采用高效液相制备色谱获得了高纯度(95.2%)的绿原酸。所建立的绿原酸制备工艺操作简便、高效环保,产品功效明确,具有良好的市场应用前景。The separation and purification processes of chlorogenic acid from green coffee beans were studied. On the basis of single-factor experiments, hot water extraction of ehlorogenic acid was optimized by response surface methodology. The results showed that the chlorogenie acid yield was significantly affected by the four factors, and the optimum extraction conditions were temperature 79.8 ℃, solvent pH 2.8, solid/liquid ratio 1：6 and extraction time 3.0 h. The maximum extraction yield of chlorogenic acid was 92.0%. The experiments on screening of different resins showed that XDA-8 resin had best efficiency in ehlorogenic acid separation and purification. Under the optimized conditions, chlorogenic acid content and recovery reached 70.9% and 85.4%, respectively. Preparative high performance liquid chromatography was used to obtain high purity （95.2%） of chlorogenie acid from green coffee bean extract. The established preparation process of chlorogenie acid had the advantages of simple, efficient and environmentally friendly.

关 键 词：绿原酸 绿咖啡豆 提取 纯化 树脂 

分 类 号：Q946.81[生物学—植物学]