Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration  

作　　者：Guang-bin Zha Mi Shen Xiao-song Gu Sheng Yi 

机构地区：[1]Jiangsu Key Laboratory of Neuroregeneration,Co-innovation Center of Neuroregeneration,Nantong University

出　　处：《Neural Regeneration Research》2016年第9期1506-1511,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81130080,31300942;the National Key Basic Research Program of China(973 Program);No.2014CB542202;the Natural Science Foundation of Jiangsu Province,China,No.BK20150409;the Natural Science Foundation of Jiangsu Higher Education Institutions of China,No.15KJB180013;the Scientific Research Foundation of Nantong University of China,No.14R29;the Natural Science Foundation of Nantong City in China,No.MS12015043;the Priority Academic Program Development of Jiangsu Higher Education Institutions of China

摘　　要：Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, wheth- er tau is involved in peripheral nerve regeneration remains unknown. In the current study, we obtained sciatic nerve tissue from adult rats 0, 1, 4, 7, and 14 days after sciatic nerve crush and examined tau mRNA and protein expression levels and the location of tau in the sciatic nerve following peripheral nerve injury. The results from our quantitative reverse transcription polymerase chain reaction analysis showed that compared with the uninjured control sciatic nerve, mRNA expression levels for both tau and tau tubulin kinase 1, a serine/ threonine kinase that regulates tau phosphorylation, were decreased following peripheral nerve injury. Our western blot assay results suggested that the protein expression levels of tau and phosphorylated tau initially decreased 1 day post nerve injury but then gradually increased. The results of our immunohistochemical labeling showed that the location of tau protein was not altered by nerve injury. Thus, these results showed that the expression of tau was changed following sciatic nerve crush, suggesting that tau may be involved in periph- eral nerve repair and regeneration.Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, wheth- er tau is involved in peripheral nerve regeneration remains unknown. In the current study, we obtained sciatic nerve tissue from adult rats 0, 1, 4, 7, and 14 days after sciatic nerve crush and examined tau mRNA and protein expression levels and the location of tau in the sciatic nerve following peripheral nerve injury. The results from our quantitative reverse transcription polymerase chain reaction analysis showed that compared with the uninjured control sciatic nerve, mRNA expression levels for both tau and tau tubulin kinase 1, a serine/ threonine kinase that regulates tau phosphorylation, were decreased following peripheral nerve injury. Our western blot assay results suggested that the protein expression levels of tau and phosphorylated tau initially decreased 1 day post nerve injury but then gradually increased. The results of our immunohistochemical labeling showed that the location of tau protein was not altered by nerve injury. Thus, these results showed that the expression of tau was changed following sciatic nerve crush, suggesting that tau may be involved in periph- eral nerve repair and regeneration.

关 键 词：nerve regeneration sciatic nerve crush microtubule-associated protein TAU phosphorylated tau （Ser 404） tau hyper-phosphorylation tau tubulin kinase 1 microtubule structure microtubule assembly and disassembly peripheral nervous system neural regeneration 

分 类 号：R745[医药卫生—神经病学与精神病学]