羚牛线粒体基因组全序列测定与分析  

Analysis on Complete Mitochondrial Genome Sequence of Golden Takin(Budorcas taxicolor)

作　　者：冯慧[1,2] 冯成利[1] 黄原[2] 王璐[1] Feng Hui Feng Chengli Huang Yuan Wang Lu(Shaanxi Institute of Zoology, Xi＇an, 710032 Shaanxi Normal University, Xi＇an, 710062)

机构地区：[1]陕西省动物研究所,西安710032 [2]陕西师范大学,西安710062

出　　处：《基因组学与应用生物学》2016年第9期2299-2305,共7页Genomics and Applied Biology

基　　金：陕西省13115重大科技专项陕西国家兽类保护动物物种鉴别系统的构建(2010ZDKG-54);陕西省科技计划项目秦岭羚牛基因特征及遗传多样性研究(2012KW-21);陕西省科学院青年人才培养项目秦岭有蹄类保护动物遗传多样性及其变化研究(2009K-02)共同资助

摘　　要：利用长距离PCR扩增法对秦岭羚牛线粒体基因组进行测序、拼接、注释并对基因组特点和羚牛系统发育进行分析。结果表明,羚牛线粒体基因组全长为16662 bp,GenBank序列号:KU361169。该基因由13个蛋白编码基因、22个转运RNA基因、2个核糖体RNA基因和1个非编码控制区组成。基因组核苷酸组成为A:33.9%,T:27.0%,C:26.3%,G:12.8%,(A+T)含量(60.9%)高于(G+C)含量(39.1%),有一定的碱基偏好。ND2、ND3、ND5基因起始密码子为ATA,其余10个蛋白质编码基因起始密码子为ATG。ND2、ND3、ND4、COⅢ基因为不完全终止密码子T,其余蛋白质基因为完全终止密码子TAA和AGA。预测了22个t RNA二级结构,共有38处错配,以GU错配为主。基于14种有蹄类动物线粒体基因组构建的系统发生树,表明羚牛与山羊亲缘关系最近,应归为羊亚科。The complete mitochondrial genome sequence of Budorcas taxicolor was determined, spliced and annotated by long PCR. The phylogenetic relationship ofBudorcas taxicolor was analyzed. The results showed that the total length of mitochondrial genome of Budorcas taxicolor was 16 662 bp （the accession number of GenBank： KU361169）. The mitogenome contained 13 protein coding nucleotide composition was A： 33.9%, T： 27.0%, C： 26.3%, G genes, 22 tRNA, 2 rRNA, and a control region. The 12.8%, （A＋T） （60.9%） was higher than （G＋C） （39.1%）. There was a strong A-T bias. The mitochondrial protein coding genes ND2, ND3 and ND5 used ATA as initiation codon, other 10 protein coding genes used ATG. The ND2, ND3, ND4 and CO HI genes had in-complete termin- ation codon （T）, while others used termination codons （TAA and AGA） as usual. The secondary structures of 22 tRNAs were predicted. There were 38 mismatch most of which were GU mismatch. The BI phylogenetic tree was built by the complete mitochondrial genome sequences of 14 Ungulata animals. The relationship of Budorcas taxicolor and Capra aegagrus hircus was closest. The result supported Budoreas taxieolor should be placed into Caprinae.

关 键 词：羚牛 线粒体基因组 RNA二级结构 系统进化 

分 类 号：Q953[生物学—动物学]