基于ORAC评价多酚类化合物抗氧化活性  

The Evaluation of Antioxidant Activity of Polyphenol Compounds Using ORAC

作　　者：常强[1,2] 苏明华[1,2] 陈清西[1] 曾碧玉[1,2] 王伟[1,2] 李惠华[2] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建省亚热带植物研究所,福建省亚热带植物生理生化重点实验室,福建厦门361006

出　　处：《亚热带植物科学》2016年第3期225-229,共5页Subtropical Plant Science

基　　金：厦门市科技计划资助项目(3502Z20142001)

摘　　要：采用氧自由基清除能力(ORAC)方法考察20种多酚类化合物的抗氧化活性。结果表明,该方法具有较优的线性关系(R^2=0.997);检测限(LOD)和定量限(LOQ)为0.5~3.1μmol·L^(-1),精密度<18%,准确度91%~105%。比较20种多酚类化合物抗氧化活性,对羟基苯甲酸类化合物中,鞣花酸和没食子酸具有较强的抗氧化活性;对羟基肉桂酸类以咖啡酸及其衍生物抗氧化活性最高;在类黄酮组分中,黄烷-3-醇表现出优良的抗氧化特性,黄酮醇次之。ORAC可作为评价多酚类化合物抗氧化的简便、高效的标准化检测方法。The oxygen radical absorbance capacity （ORAC） method was validated, and then ORAC values of selected 20 phenolic compounds were determined. The results indicated that linearity （R^2 =0.997）, limit of detection and limit of quantification （0.5-3.1 μmol·L^-1）, precision （〈18%）, accuracy （91%-105%） were determined. And ellagic acid and gallic acid had strong antioxidant activity in p-hydroxy benzoic acid compounds. Caffeic acid and its derivatives had highest antioxidant activity in hydroxy cinnamic acid. In the flavonoid constituents, flavan-3-ols showed good antioxidant properties, followed by flavonol. ORAC was considered as simple, efficient detection method of standardization for evaluation of antioxidant activity of the phenolic compounds.

关 键 词：氧自由基清除能力 抗氧化活性 多酚 

分 类 号：Q946-33[生物学—植物学]