Annexin A7在匹罗卡品癫痫小鼠模型脑组织中的表达  

Expression of Annexin A7 in brain tissue of pilocarpine-induced epilepsy mouse model

作　　者：贾俊伟[1] 周雪颖[2] 李秋红[1] 于凯[1] 周国钰[3] 周盛年[1] 

机构地区：[1]山东大学齐鲁医院神经内科山东大学脑科学研究所 [2]山东大学齐鲁医院康复医学科 [3]山东大学齐鲁医院干部保健科,山东济南250012

出　　处：《山东大学学报（医学版）》2016年第10期6-10,45,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81371439)

摘　　要：目的探讨Annexin A7在匹罗卡品癫痫小鼠模型脑组织中的表达。方法将30只健康成年雄性C57BL/6小鼠随机分为实验组(n=20)和对照组(n=10),实验组建立癫痫小鼠模型,观察两组行为学特点,分别运用蛋白质免疫印迹和免疫荧光标记技术检测Annexin A7在两组小鼠脑组织中的表达。结果实验组20只小鼠中有18只出现了癫痫持续状态,最终存活8只,其中有6只出现自发性发作,对照组均未出现癫痫持续状态和自发性发作。实验组海马和颞叶皮质中Annexin A7的表达均显著高于对照组(P<0.05);实验组海马和颞叶皮质中Annexin A7表达于神经元,而不表达于星形胶质细胞。结论 Annexin A7可能参与癫痫的发生与发展。Objective To explore the expression of Annexin A7 in brain tissue of pilocarpine-induced epilepsy mouse model. Methods Thirty healthy adult male C57 BL /6 mice were randomly divided into experimental group（ n = 20）and control group（ n = 10）. Epilepsy mouse model was established in experimental group and behavioural characteristics of mice were observed in two groups. Expression of Annexin A7 was detected using Western blotting and immunofluorescence labeling techniques. Results Eighteen mice suffered from status epilepticus and only eight survived in the experimental group. Of which,six mice encountered spontaneous seizures in experimental group. Status epilepticus and spontaneous seizures did not occur in control group. Expression of Annexin A7 in the hippocampus and temporal cortex of experimental group were markedly higher than that of control group（ P〈0. 05）. In the brain tissue of epilepsy mouse model,Annexin A7 was expressed in the neurons rather than astrocytes. Conclusion Annexin A7 may be involved in the occurrence and development of epilepsy.

关 键 词：癫痫 ANNEXIN A7 小鼠 匹罗卡品 脑组织 

分 类 号：R742.1[医药卫生—神经病学与精神病学]