IQGAP1通过经典Wnt通路促进非小细胞肺癌恶性表型  

IQGAP1 promotes the malignant phenotype of NSCLC via the canonical Wnt pathway

作　　者：赵环宇[1] 韩阳[1] 杜江[1] 韩勇[1] 王恩华[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,附属第一医院病理科,辽宁沈阳110001

出　　处：《解剖科学进展》2016年第5期507-509,512,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81272606)

摘　　要：目的探讨非小细胞肺癌细胞中IQ-domain GTPase-activatingprotein 1(IQGAP1)与Dishevelled(Dvl)的相关性。方法应用免疫组化法检测109例非小细胞肺癌组织中IQGAP1和Dvl的表达,分析与临床病理因素的关联。结果 IQGAP1在肺癌组织中的表达水平显著高于癌旁正常组织。IQGAP1与Dvl在肺癌细胞浆及核的共定位表达显著正相关,而核共定位组的c-myc和cyclin D1表达率明显高于浆共定位组(P<0.05)。淋巴结转移组中二者浆/核共定位表达率明显高于未转移组(P<0.05),且与生存时间呈正比。结论 IQGAP1可能在胞浆促进Dvl入核从而激活经典Wnt通路,促进市癌的恶性表型。Objective To investigate the relationship between IQ-domain GTPase-activating protein 1 （IQGAP1） and Dishevelled （Dvl） in human non-small cell lung cancer（NSCLC）. Methods The expression of IQGAP1 and Dvl in 109 cases of NSCLC was detected by immunohistochemistry, and the correlation between their expression and the clinical pathological parameters was analyzed. Results IQGAPI expression level was significantly higher in NSCLC than in normal adjacent tissue of hmg （ P〈 0.05）. The coexpmssion of IQGAP1 and Dvl in the cytoplasm and nucleus was sigrfificantly correlated, and the positive expression rates of c-mye and cyclin D1 were significantly higher in nuclear coexpression than in cytoplasmic coexpression （P 〈 0.05）. The coexpression rate of IQGAP1 and Dvl in the cytoplasm and nucleus was significantly higher in lymph nodal metastases than in primary growths, significantly correlated with the survival time. Condusion The promotion of the malignant phenotype of NSCLC by IQGAP1 might be related to the activation of the canonical Wnt pathway via modulating Dvl nuclear translocation from cytoplasm.

关 键 词：IQGAP1 DVL 免疫组化 人非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]