表没食子酸酯诱导卵巢癌SKOV3细胞凋亡及机制的研究  

EGCG Induce the Apoptosis of Ovarian Cancer SKOV3 Cells and Its Mechanism

作　　者：周菲菲[1] 董天睿[1] 王晶[1] 刘艳秋[2] 倪锦红[1] ZHOU Fei-fei DONG Tian-rui WANG Jing LIU Yan-qiu NI Jin-hong(Department of Gynecology, The First Affiliated Hospital of Harbin Medical University,Harbin 150001 ,China Hong Xinglong Center Hospital, Harbin 150001, China)

机构地区：[1]哈尔滨医科大学附属第一医院妇科,150001 [2]红兴隆中心医院

出　　处：《国际妇产科学杂志》2016年第5期539-541,共3页Journal of International Obstetrics and Gynecology

摘　　要：目的:探讨表没食子酸酯(EGCG)对人卵巢癌SKOV3细胞凋亡的影响及其作用机制。方法:SKOV3细胞分为4组,即阴性对照组(以正常培养液培养)、EGCG低浓度组(1μmol/L EGCG)、EGCG中浓度组(10μmol/L EGCG)和EGCG高浓度组(100μmol/L EGCG)。应用四甲基偶氮唑盐(MTT)比色法测定各组SKOV3细胞的增殖抑制率,Hoechs染色观察细胞核凋亡,电镜观查细胞形态,蛋白质印迹(Western blot)检测Caspase-3表达。结果:细胞增殖抑制实验结果显示,EGCG低、中、高浓度组SKOV3细胞的增殖率为(81.33±3.71)%、(67.00±7.21)%和(54.67±3.84)%,与阴性对照组(101.00±3.06)%比较差异具有统计学意义(P<0.05)。Hoechst33258染色结果显示,EGCG低、中、高浓度组凋亡细胞比例分别为(16.77±2.48)%、(24.33±3.70)%和(39.67±4.11)%,与阴性对照组[(2.00±1.45)%]相比差异均有统计学意义(P<0.05)。电镜观察结果显示,随着EGCG作用浓度的增加,SKOV3细胞超微结构损害程度逐渐明显。此外,EGCG可以升高Caspase-3表达水平。结论:EGCG通过增强Caspase-3表达诱导卵巢癌SKOV3细胞凋亡。Objective：To investigate the effect and mechanism of EGCG on human ovarian cancer SKOV3 cells′apoptosis. Methods：SKOV3 cells were divided into four groups. Negative control group：cultured with normal culture medium;EGCG low dose group：cultured with 1μmol/L EGCG;EGCG middle dose group：cultured with 10μmol/L EGCG;EGCG high dose group：cultured with 100μmol/L EGCG. Cell proliferation inhibition rate of each group of SKOV3 cells were test by MTT assay, nuclei apoptosis were checked by Hoechs staine, cells morphology were investigated by electron microscope, Caspase-3 expression was detected by Western-blot. Results：Cell proliferation inhibition test results showed that the inhibition rate of EGCG low, medium and high concentration group on SKOV3 cells was （81.33±3.71）%, （67.00±7.21）%and （54.67±3.84）%, the difference was statistically significant （P〈0.05） compared with the negative control group （101.00±3.06）%. Hoechst33258 staining results showed that, apoptosis cell percentage of EGCG low, medium and high concentration group was （16.77 ±2.48）%, （24.33±3.70）%and （39.67±4.11）%, the difference was statistically significant （P〈0.05） compared with the negative control group [（2.00±1.45）%]. Electron microscopy showed that with the concentration of EGCG elevated, the damage on ultrastructure of SKOV3 cell became clear. In addition, EGCG could raised Caspase-3 expression levels. Conclusions：EGCG induced ovarian cancer SKOV3 cells apoptosis by enhancing the expression of Caspase-3 gene.

关 键 词：表没食子酸酯 没食子酸 儿茶素 卵巢肿瘤 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 

分 类 号：R285[医药卫生—中药学]