Gibberella intermedia WX12产特异性糖苷酶的发酵工艺优化  

Optimization of Culture Conditions for Specific Production of Glycosidase from Gibberella intermedia WX12

作　　者：杨南南[1] 李会[1] 吴燕[1] 张晓梅[1] 许正宏[1] 史劲松[1] 

机构地区：[1]江南大学药学院,江苏无锡214122

出　　处：《食品与生物技术学报》2016年第10期1028-1034,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(21206055);国家863计划项目(2011AA02A211)

摘　　要：赤霉菌(Gibberella intermedia WX12)糖苷酶能将黄姜薯蓣皂苷转化为薯蓣皂苷元。先后采用单因素实验和正交实验对G.intermedia WX12发酵产糖苷酶的培养基组分和发酵工艺进行了优化。确定最佳发酵工艺条件为:葡萄糖5 g/L,酵母粉25 g/L,NaCl 1.16 g/L,KH_2PO42.72 g/L,MgSO_20.3 g/L,培养基初始pH 6.0,接种量为体积分数8%。G.intermedia WX12在上述条件下,于220 r/min、30℃摇床培养96 h,糖苷酶活性达到28.1 U/m L,较优化前提高4倍。在此基础上利用粗酶液对黄姜薯蓣皂苷进行转化,50℃条件下转化12 h后,薯蓣皂苷元转化率可达到60%。A glycosidase from Gibberella intermedia WX12 could convert dioscin into diosgenin. Through the single factor and orthogonal experiments,the optimal fermentation medium was found to consist of 5 g/L glucose,25 g/L yeast,1.16 g/L NaCl,2.72 g/L KH2PO4 and 0.3 g/L MgSO4 and the culture parameters for dioscin-glycosidase production were temperature 30 ℃,rotation speed 220 r/min,initial pH 6.0 and inoculum size 8.0%（v/v）. With these conditions,the enzyme production had a similar trend to that of cell growth curve,and the highest activity of glycosidase reached 28.1 U/mL after fermentation for 96 h,which was 5 times higher than that before optimization. A conversion rate of 60% was achieved when the obtained crude enzyme was used to convert dioscin from Dioscorea zingiberensis C.H. Wright into diosgenin at 50 ℃ for 12 h.

关 键 词：薯蓣皂苷元 糖苷酶 优化 赤霉菌 

分 类 号：TQ925[轻工技术与工程—发酵工程]