紫色红曲霉Mp-24高产Monacolin K发酵工艺响应面优化  

Optimizing fermentation conditions of high-yielding Monacolin K by Monascus purpureus strain Mp-24 using response surface methodology

作　　者：Bedelkhan Almagul 任浩[1] 章婷[1] 郑婕施[1] 韩肖飞[1] 蒋冬花[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《工业微生物》2016年第5期23-30,共8页Industrial Microbiology

基　　金：国家自然科学基金项目(NO.31270061;NO.31570013)

摘　　要：将单因素实验结果与响应面法相结合,对高产Monacolin K的紫色红曲霉Mp-24菌株进行发酵工艺条件优化。通过摇瓶发酵对碳源、氮源、碳源含量、氮源含量、培养时间等进行单因素优化,确定Mp-24菌株摇瓶发酵适宜条件:乳糖为碳源、酵母膏为氮源、碳源含量7%、氮源含量2%、培养时间12 d,Monacolin K产量为167 mg/L。应用Box-Behnken中心组合试验设计建立数学模型,进行响应面分析优化发酵条件,结果显示最佳发酵工艺条件为:碳源(乳糖)8%,氮源(酵母膏)3%,培养时间11 d,在此条件下Monacolin K的含量达到247.8 mg/L,比优化前提高1.5倍。Response surface methodology and single factor experiment were combined to optimize the submerged culture conditions for the production of Monacolin K using Monascus purpureus strain Mp-24. Through analyzing the influence of carbon sources,nitrogen sources,carbon content,nitrogen content and culture time,the results showed that optimal fermentation conditions were as follows： lactose（ carbon source）,yeast extract（ nitrogen source）,carbon content of 7%,nitrogen content of 2% and culture time of 12 d. Monacolin K yield was 167 mg/L. After using Box-Behnken central composite design and response surface analysis,the optimum conditions were： carbon（ lactose） of 8%,nitrogen（ yeast extract） of 3% and culture time of 11 d. Under the optimized conditions,the content of Monacolin K reached 247. 8 mg/L.The optimized fermentation conditions resulted in 1. 5 times increase of Monacolin K yield in comparison with that of the original conditions.

关 键 词：紫色红曲霉 Monacolin K 发酵工艺 应面优化 

分 类 号：TQ925.7[轻工技术与工程—发酵工程]