若羌灰枣多糖提取工艺优化研究  

Optimization of Extraction Process of polysaccharides from Ruoqiang grey jujube

作　　者：魏金凤[1,2,3] 贾玉华[1,2,3] 闫静[1] 曹鹏然 雷璐倩 胡志宇[1] 康文艺[1,2] 

机构地区：[1]河南大学中药研究所,河南开封475004 [2]开封市保健食品功效成分研究重点实验室,河南开封475004 [3]河南大学民生学院,河南开封475004

出　　处：《河南大学学报（医学版）》2016年第3期167-171,共5页Journal of Henan University:Medical Science

基　　金：2016年度河南省高校科技创新团队药食两用资源研究开发项目(16IRTSTHN019);河南省科技厅基础前沿计划(142300410123;152300410064);开封市创新人才项目(1509010);河南大学民生学院教育教学改革研究项目(MSJG2015007;MSJG2015040);河南大学2016年度大学生创新创业训练计划项目(16NA058)

摘　　要：〔目的〕利用响应面分析法对新疆若羌灰枣多糖的提取进行优化,考察提取温度、时间、液料比对多糖提取率的影响。〔方法〕在单因素试验的基础上选取试验因素与水平,根据Box-Behnken试验设计原理,确定其最佳提取工艺条件为:提取时间1.68 h,提取温度78.27℃,液料比27.36∶1。〔结果〕在此最佳工艺条件下,枣多糖的提取率可达71.19%。〔结论〕在此基础上采用Sevage法除去枣多糖溶液中蛋白质,在样品、Sevage为1∶1,氯仿、正丁醇为4∶1的条件下,研究萃取次数对蛋白质和多糖的影响,得出最佳萃取次数为6次。〔Objective〕This paper aimed at optimization of the extraction of Ruoqiang grey jujube polysaccharides by Response Surface Methodlogy, the study investigated extracting temperature, time and liquid ratio on the polysaccharide extraction yield.〔Methods〕On the basis of the previous results and according to the principle of Box-Behnken experiment design, the results showed that the best extraction technology conditions were determined as follows： extraction time 1.68 h, extraction temperature78.27 ℃, liquid ratio of 27. 36 ∶ 1.〔Results〕Under optimum conditions, the extraction rate of polysaccharides up to 71. 19%.〔Conclusion〕On this basis of extract, jujube polysaccharides solution using sevage method eliminating the protein, under the conditions of the ratio of sample Sevage is 1 ∶ 1, and the ratio of chloroform n-butanol is 4 ∶ 1, studied the extraction times effect of protein and polysaccharides, finding the best extracted six times.

关 键 词：多糖提取 响应面分析 脱蛋白 

分 类 号：R28[医药卫生—中药学]