蛹虫草多糖和D-甘露醇深层发酵的非结构动力学模型  

Unstructured kinetic models for production of polysaccharides and D-mannitol by Cordyceps militaris in submerged fermentation

作　　者：王晓玲[1,2] 周广乙 刘高强[1,2] 

机构地区：[1]中南林业科技大学经济林培育与保护省部共建教育部重点实验室,湖南长沙410004 [2]中南林业科技大学林业生物技术湖南省重点实验室,湖南长沙410004

出　　处：《菌物学报》2016年第10期1250-1257,共8页Mycosystema

基　　金：教育部"新世纪优秀人才支持计划"基金(NCET-13-1046);湖南省高校学科带头人培养对象基金(2014-04);湖南省高校创新平台开放基金(12K069;14K112)~~

摘　　要：多糖和D-甘露醇是蛹虫草的重要药理活性成分。本文开展了蛹虫草在分批发酵过程中同时生产多糖和D-甘露醇的发酵动力学研究。利用Sigmoid函数构建了蛹虫草菌丝生长、糖基质消耗、多糖和D-甘露醇的非结构动力学模型,并根据Boltzmann方程拟合求解出各模型参数。结果显示,各模型的实测值和预测值拟合度较好。蛹虫草比生长速率在第1.0天达到最大值(μmax)1.244d^(-1);底物葡萄糖的比消耗速率在第0.6天达到最大值(qS,max)2.163d^(-1);多糖比合成速率在第2.0天达到最大值(qP,max)51.852mg/(g?d);D-甘露醇比合成速率在第0.99天左右达到最大值(qD,max)37.963mg/(g?d)。蛹虫草多糖的形成与菌丝细胞的生长呈现部分生长关联型,而D-甘露醇的形成与细胞生长呈现生长关联型关系。研究结果为利用分批发酵规模化同时发酵生产蛹虫草多糖和D-甘露醇提供了理论依据。Polysaccharides and D-mannitol are two of the key physiologically active components of Cordyceps militaris. The dynamic characters of simultaneous production of polysaccharides and D-mannitol by C. militaris in batch fermentation process were described. Unstructured kinetics of mycelial cell growth, glucose consumption, polysaccharides and D-mannitol were investigated based on Sigmoid model. The parameters of the model were determined by Boltzmann simulation, and the tested values matched well with the values predicted by the models. The maximum specific mycelial cell growth rate（μmax）, the maximum specific glucose consumption rate（qS, max）, the maximum specific polysaccharide biosynthesis rate（qP, max） and the maximum specific D-mannitol biosynthesis rate（qD, max） were 1.244d-1, 2.163d-1, 51.852mg/（g？d） and 37.963mg/（g？d）, respectively, and the maximum values were obtained in 1.0d, 0.6d, 2.0d and 0.99 d, respectively. Polysaccharide production assumed partial growth-association mode, while D-mannitol biosynthesis growth-association mode. These results provide useful information for simultaneous production of polysaccharides and D-mannitol by C. militaris in batch fermentation process in a large scale.

关 键 词：药用真菌 液体发酵 虫草酸 活性成分 发酵动力学 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]